研究 RNA-蛋白质相互作用的方法。
Methods to study RNA-protein interactions.
机构信息
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA, USA.
Veterans Affairs Palo Alto Healthcare System, Palo Alto, CA, USA.
出版信息
Nat Methods. 2019 Mar;16(3):225-234. doi: 10.1038/s41592-019-0330-1. Epub 2019 Feb 25.
Noncoding RNA sequences, including long noncoding RNAs, small nucleolar RNAs, and untranslated mRNA regions, accomplish many of their diverse functions through direct interactions with RNA-binding proteins (RBPs). Recent efforts have identified hundreds of new RBPs that lack known RNA-binding domains, thus underscoring the complexity and diversity of RNA-protein complexes. Recent progress has expanded the number of methods for studying RNA-protein interactions in two general categories: approaches that characterize proteins bound to an RNA of interest (RNA-centric), and those that examine RNAs bound to a protein of interest (protein-centric). Each method has unique strengths and limitations, which makes it important to select optimal approaches for the biological question being addressed. Here we review methods for the study of RNA-protein interactions, with a focus on their suitability for specific applications.
非编码 RNA 序列，包括长非编码 RNA、小核仁 RNA 和非翻译 mRNA 区域，通过与 RNA 结合蛋白 (RBP) 的直接相互作用来实现其许多不同的功能。最近的研究已经确定了数百种新的 RBP，它们缺乏已知的 RNA 结合结构域，从而突出了 RNA-蛋白复合物的复杂性和多样性。最近的进展扩大了研究 RNA-蛋白相互作用的方法的数量，分为两类：一类是描述与感兴趣的 RNA 结合的蛋白质的方法（以 RNA 为中心），另一类是研究与感兴趣的蛋白质结合的 RNA 的方法（以蛋白质为中心）。每种方法都有其独特的优缺点，因此选择适合所研究的生物学问题的最佳方法非常重要。在这里，我们将综述研究 RNA-蛋白相互作用的方法，重点介绍它们在特定应用中的适用性。
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