Mianlengeh Zeinab Esmailzadeh, Najafabadi Masood Soltani, Saidi Abbas, Askari Hossein
Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, G.C, Tehran, Iran.
Genetic Research Department, and Iranian National Plant Gene Bank, Seed and Plant Improvement Institute (SPII), Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.
Iran J Biotechnol. 2018 May 15;16(2):e1422. doi: 10.21859/ijb.1422. eCollection 2018 May.
Sunflower ( L.) is one of the important vegetable oil supplies in the world and in Iran, as well. It is classified as a drought semi-tolerant crop; however, its yield is adversely affected by drought stress. Understanding the initial events in sensing stress and the related physiologic and biochemical events thereafter, is crucial in designing drought stress breeding programs. Transcription factors are master molecules directly involved in the plant responses under drought stress, from signal perception and transduction to the regulation of physiologic processes.
The expression pattern of some bZip transcription factors in response to osmotic stress was investigated in sunflower.
Employing real-time PCR to monitor, the response of 10 bZIP transcription factors was performed under different osmotic stress conditions including -0.3, 0.9, and 1.2 MPa. Whole seedling was sampled at 6, 12, and 24 h after the osmotic condition application.
Exposure to osmotic potential of 0.9 MPa for 24 h caused a reduction in the fresh weight of the seedling. Among the evaluated genes, eight genes, , , , , , , , and appeared as the osmotic stress responsive transcription factor. Changes in the expression of the genes under 0.3 MPa was observed for four genes. Most of the osmotic responsive genes appeared to be up-regulated. Most of responsiveness in the gene expression was happened under 0.9 MPa of the osmotic stress which is corresponding to fresh weight reduction in the seedlings. Among the investigated genes, two genes was identified to have possible roles in sensitive response of sunflower against drought stress.
It was a focus to have systemic view on the complex response of the plant to abiotic stress, and avoidance of the single gene analysis. Also, the importance of molecular data in molecular breeding procedures toward achievement of the stress tolerant lines was highlighted.
向日葵（L.）是世界以及伊朗重要的植物油供应来源之一。它被归类为耐旱性一般的作物；然而，其产量会受到干旱胁迫的不利影响。了解感知胁迫的初始事件以及随后相关的生理和生化事件，对于设计干旱胁迫育种计划至关重要。转录因子是直接参与植物在干旱胁迫下反应的关键分子，从信号感知和转导到生理过程的调节。
研究向日葵中一些bZip转录因子对渗透胁迫的表达模式。
采用实时PCR进行监测，在包括-0.3、0.9和1.2 MPa的不同渗透胁迫条件下，对10个bZIP转录因子的反应进行检测。在施加渗透条件后的6、12和24小时对全株幼苗进行采样。
在0.9 MPa渗透势下处理24小时导致幼苗鲜重降低。在评估的基因中，8个基因， ， ， ， ， ， ， 和 表现为渗透胁迫响应转录因子。在0.3 MPa下观察到4个基因的表达发生变化。大多数渗透响应基因似乎上调。基因表达中的大多数响应发生在0.9 MPa的渗透胁迫下，这与幼苗鲜重降低相对应。在研究的基因中，鉴定出两个基因可能在向日葵对干旱胁迫的敏感反应中起作用。
重点是对植物对非生物胁迫的复杂反应有系统的认识，避免单一基因分析。此外，强调了分子数据在分子育种程序中实现耐胁迫品系方面的重要性。
