半定量双重 RT-PCR 显示，来自美国的诊断样本中低频率出现猪革留病毒和非典型猪瘟病毒。
Semi-quantitative duplex RT-PCR reveals the low occurrence of Porcine Pegivirus and Atypical Porcine Pestivirus in diagnostic samples from the United States.
机构信息
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
出版信息
Transbound Emerg Dis. 2019 May;66(3):1420-1425. doi: 10.1111/tbed.13154. Epub 2019 Mar 21.
Porcine Pegivirus (PPgV) and Atypical Porcine Pestivirus (APPV) are two recently identified porcine viruses. In this study, the identification of two viruses by metagenomic sequencing, and a duplex semi-quantitative RT-PCR was developed to detect these pathogens simultaneously. The PPgV strain Minnesota-1/2016 had a 95.5%-96.3% nucleotide identity and clustered with the recently identified US PPgV strains, which is a distant clade from the German PPgV strains. The APPV strain Minnesota-1/2016 shared an 87.3%-92.0% nucleotide identity with the other global APPV strains identity but only shared an 82.8%-83.0% nucleotide identity with clade II consisting of strain identified in China. Detection of both PPgV and APPV was 9.0% of the diagnostic cases. Co-infection of PPgV and APPV was identified in 7.5% of the diagnostic cases. The occurrence and genetic characterization of PPgV and APPV further enhance our knowledge regarding these new pathogens in the United States.
猪戊型肝炎病毒（PPgV）和非典型猪瘟病毒（APPV）是两种新鉴定的猪病毒。本研究通过宏基因组测序鉴定了这两种病毒，并建立了一种双靶半定量 RT-PCR 方法，用于同时检测这些病原体。PPgV 株 Minnesota-1/2016 的核苷酸同一性为 95.5%-96.3%，与最近鉴定的美国 PPGV 株聚类，与德国 PPGV 株属于不同的分支。APPV 株 Minnesota-1/2016 与其他全球 APPV 株的核苷酸同一性为 87.3%-92.0%，但与中国鉴定的株组成的 II 分支仅具有 82.8%-83.0%的核苷酸同一性。两种病毒的检测率为诊断病例的 9.0%。在诊断病例中，PPgV 和 APPV 的混合感染率为 7.5%。PPgV 和 APPV 的发生和遗传特征进一步增强了我们对美国这些新病原体的认识。
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