近年来，细菌三联外排泵和 I 型分泌系统组装的模式发生了转变。
Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system.
机构信息
Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Center for Food and Bioconvergence, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Unit on Structural and Chemical Biology of Membrane Proteins, Cell Biology and Neurobiology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20892, USA.
出版信息
J Microbiol. 2019 Mar;57(3):185-194. doi: 10.1007/s12275-019-8520-1. Epub 2019 Feb 26.
Tripartite efflux pumps and the type I secretion system of Gram-negative bacteria are large protein complexes that span the entire cell envelope. These complexes expel antibiotics and other toxic substances or transport protein toxins from bacterial cells. Elucidating the binary and ternary complex structures at an atomic resolution are crucial to understanding the assembly and working mechanism. Recent advances in cryoelectron microscopy along with the construction of chimeric proteins drastically shifted the assembly models. In this review, we describe the current assembly models from a historical perspective and emphasize the common assembly mechanism for the assembly of diverse tripartite pumps and type I secretion systems.
三组分外排泵和革兰氏阴性菌的 I 型分泌系统是横跨整个细胞包膜的大型蛋白质复合物。这些复合物可将抗生素和其他有毒物质排出细菌细胞，或运输蛋白毒素。阐明二元和三元复合物的原子分辨率结构对于理解其组装和工作机制至关重要。近年来，冷冻电子显微镜技术的进步以及嵌合蛋白的构建极大地改变了组装模型。在这篇综述中，我们从历史角度描述了当前的组装模型，并强调了不同的三组分外排泵和 I 型分泌系统的共同组装机制。
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