比较分析锯缘青蟹卵巢和精巢中的 piRNA。

Comparative profiling of ovarian and testicular piRNAs in the mud crab Scylla paramamosain.

机构信息

Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.

Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China.

出版信息

Genomics. 2020 Jan;112(1):323-331. doi: 10.1016/j.ygeno.2019.02.012. Epub 2019 Feb 23.

DOI:10.1016/j.ygeno.2019.02.012

PMID:30807818

Abstract

PIWI-interacting RNAs (piRNAs) are abundantly found in germ cells and involved in gametogenesis and gonadal development. Information on the regulatory roles of piRNAs in crustacean reproduction, however, is scarce. Thus, we identified gonadal piRNAs of mud crab Scylla paramamosain. Of the 115,491 novel piRNAs, 596 were differentially expressed. Subsequently, 389,887 potential piRNA-target genes were predicted. The expression of 4 piRNAs and 9 genes with high piRNA interactions were validated with the inclusion of additional immature specimens, including LRP2 that is involved in growth and reproduction, MDN1 in ribosome biogenesis pathway and gametogenesis, and PRKDC, a DNA repair gene involved in gonadal differentiation and maturation. KEGG analysis further revealed the involvement of predicted piRNA target genes in gametogenesis- and reproduction-related pathways. Our findings provide baseline information of mud crab piRNAs and their differential expression between testes and ovaries suggests that piRNAs play an essential role in regulating gametogenesis and gonadal development.

摘要

PIWI 相互作用 RNA（piRNAs）在生殖细胞中大量存在，参与配子发生和性腺发育。然而，关于 piRNAs 在甲壳动物生殖中的调控作用的信息很少。因此，我们鉴定了泥蟹 Scylla paramamosain 的性腺 piRNAs。在 115491 个新的 piRNAs 中，有 596 个表现出差异表达。随后，预测了 389887 个潜在的 piRNA 靶基因。包含额外的未成熟标本后，验证了 4 个 piRNAs 和 9 个具有高 piRNA 相互作用的基因的表达，包括参与生长和生殖的 LRP2、核糖体生物发生途径和配子发生的 MDN1，以及参与性腺分化和成熟的 DNA 修复基因 PRKDC。KEGG 分析进一步表明，预测的 piRNA 靶基因参与了与配子发生和生殖相关的途径。我们的研究结果提供了泥蟹 piRNAs 的基础信息，以及它们在睾丸和卵巢之间的差异表达，表明 piRNAs 在调节配子发生和性腺发育中发挥着重要作用。