Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China.
Sci Rep. 2019 Feb 26;9(1):2819. doi: 10.1038/s41598-019-39205-2.
In this study, we investigated the possibility of rearing and breeding the razor clam (Sinonovacula constricta) in inland low salinity water or freshwater. Long-term low salinity (LS) rearing was performed for 3 months to determine the effects of LS on the survival rate, growth rate, and the activities of critical enzymes in juvenile S. constricta (JSC). The survival rate in the LS group was only 15.67% at the end of the LS rearing test. In the first month, the survival rate in the LS group was significantly lower than that in the control group (P < 0.001). The growth rate (shell length growth rate and weight gain rate) was significantly lower in the LS group than the control group in the first month (P < 0.001 for length and weight). However, the growth rates in the two groups differed little during the second and third months. The oxygen consumption and ammonia excretion rates by JSCs were significantly higher in the LS group than the control group during the first month, but they decreased gradually during the following 2 months. The Na/K ATPase and superoxide dismutase activities were significantly higher in the LS group than the control group during the first month, but they then decreased gradually until there were no significant differences between the groups. However, the aspartate aminotransferase activity was higher in the LS group than the control group during all 3 months. Most of the JSCs died due to LS but the survival of some JSCs suggests the possibility of breeding LS resistant or freshwater S. constricta.
在这项研究中，我们调查了在低盐水或淡水中养殖 razor clam（Sinonovacula constricta）的可能性。进行了为期 3 个月的长期低盐（LS）养殖，以确定 LS 对幼年 S. constricta（JSC）的存活率、生长率和关键酶活性的影响。LS 组的存活率在 LS 养殖试验结束时仅为 15.67%。在第一个月，LS 组的存活率明显低于对照组（P<0.001）。在第一个月，LS 组的生长率（壳长生长率和体重增长率）明显低于对照组（P<0.001 用于长度和重量）。然而，在第二个和第三个月，两组的生长率差异不大。在第一个月，JSCs 的耗氧量和氨排泄率在 LS 组明显高于对照组，但在随后的 2 个月中逐渐下降。在第一个月，LS 组的 Na/K ATPase 和超氧化物歧化酶活性明显高于对照组，但随后逐渐下降，直到两组之间没有显著差异。然而，天冬氨酸氨基转移酶活性在 LS 组高于对照组在所有 3 个月。大多数 JSCs 因 LS 而死亡，但一些 JSCs 的存活表明有可能繁殖 LS 抗性或淡水 S. constricta。
