Yang Min, Han Yuting, Chang Yujie, Li Chengbo, Niu Donghong
Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China.
Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China.
Mar Biotechnol (NY). 2025 Mar 26;27(2):68. doi: 10.1007/s10126-025-10445-w.
The razor clam (Sinonovacula constricta) is a key species in marine aquaculture, known for its wide salinity adaptation, and potential for cultivation in saline-alkaline water. Understanding its response mechanisms is crucial for expanding its farming into these regions. This study reveals the response mechanisms of S. constricta in response to low-salinity alkaline stress through a combined analysis of transcriptomics and metabolomics. After 24 h of salt-alkali stress (SA group), 1378 differentially expressed genes (DEGs) were identified, with enriched pathways including glycerophospholipid metabolism, serine, taurine, and hypotaurine metabolism. Additionally, 341 significantly different metabolites (SDMs) were found, primarily involved in taurine and hypotaurine metabolism, purine metabolism, and the FoxO signaling pathway, etc. Both DEGs and SDMs were notably enriched in hypotaurine metabolism, glycerophospholipid metabolism, and the mTOR signaling pathway, showing significant upregulation in the SA group. Correlation analysis found that the integrated regulatory network was involved in the synthesis of taurine, glycerophospholipids, and L-glutamic acid, and the metabolism of 3-mercaptopropionic acid. These results suggest that low salinity and alkalinity induce stress responses in S. constricta by regulating osmotic balance, phospholipid synthesis, and lipid metabolism. This study offers insights into the molecular mechanisms of salt-alkali response in S. constricta.
缢蛏（Sinonovacula constricta）是海水养殖中的关键物种，以其广泛的盐度适应性和在盐碱水中的养殖潜力而闻名。了解其响应机制对于将其养殖扩展到这些地区至关重要。本研究通过转录组学和代谢组学的联合分析，揭示了缢蛏对低盐度碱性胁迫的响应机制。在盐碱胁迫24小时后（SA组），鉴定出1378个差异表达基因（DEGs），富集的途径包括甘油磷脂代谢、丝氨酸、牛磺酸和亚牛磺酸代谢。此外，发现了341种显著不同的代谢物（SDMs），主要涉及牛磺酸和亚牛磺酸代谢、嘌呤代谢以及FoxO信号通路等。DEGs和SDMs在亚牛磺酸代谢、甘油磷脂代谢和mTOR信号通路中均显著富集，在SA组中呈显著上调。相关性分析发现，整合调控网络参与了牛磺酸、甘油磷脂和L-谷氨酸的合成以及3-巯基丙酸的代谢。这些结果表明，低盐度和碱度通过调节渗透平衡、磷脂合成和脂质代谢诱导缢蛏产生应激反应。本研究为缢蛏盐碱响应的分子机制提供了见解。
