Department of Pharmaceutical Sciences, University of Piemonte Orientale 'A. Avogadro', Via Bovio 6, 28100 Novara, Italy.
Department of Life Sciences & Systems Biology, University of Torino, via Accademia Albertina 23, 10123 Torino, Italy.
Nanomedicine (Lond). 2019 Mar;14(5):575-594. doi: 10.2217/nnm-2018-0256. Epub 2019 Feb 27.
To clarify the mechanisms of interaction between SiO nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells.
MATERIALS & METHODS: Nontoxic doses of SiO NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach.
TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca] increase.
These findings suggest that SiO NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.
阐明 SiO 纳米颗粒（NPs）与 GT1-7 神经内分泌细胞质膜相互作用的机制，重点关注钙通透性通道的激活，该通道负责这些细胞中持续的钙内流和电活动的调节。
将无毒剂量的 SiO NPs 给予细胞。钙成像和膜片钳技术与药理学方法相结合。
TRPV4、Cx 和 Panx 样通道是 NP 诱导内向电流的主要组成部分。用抗氧化剂 N-乙酰-L-半胱氨酸孵育可强烈降低[Ca]增加。
这些发现表明，SiO NPs 通过催化自由基的产生，直接激活一组复杂的钙通透性通道。
