Liang Hao, Jin Chan, Tang Ying, Wang Fude, Ma Chunwang, Yang Yongji
Physics College, Henan normal university, Xinxiang, 453007, China.
J Appl Toxicol. 2014 Apr;34(4):367-72. doi: 10.1002/jat.2953. Epub 2013 Oct 24.
Despite the widespread use of silica nanoparticles (SiO2 NPs) in biological and medical fields, their adverse effects have not been clearly elucidated. In this study, spherical SiO2 NPs with a 50 nm diameter were used to study their interaction with HaCaT cells. SiO2 NPs were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant glutathione depletion and reactive oxygen species generation, which reduced the cellular antioxidant level, could be the major factor of cytotoxicity induced by SiO2 NPs.
尽管二氧化硅纳米颗粒（SiO₂ NPs）在生物和医学领域得到了广泛应用，但其不良影响尚未得到明确阐明。在本研究中，使用直径为50 nm的球形SiO₂ NPs来研究它们与HaCaT细胞的相互作用。发现SiO₂ NPs很容易被HaCaT细胞内化并定位在细胞质、溶酶体和自噬体中。细胞活力下降和细胞膜完整性受损表明了SiO₂ NPs的细胞毒性。显著的谷胱甘肽消耗和活性氧生成，降低了细胞抗氧化水平，可能是SiO₂ NPs诱导细胞毒性的主要因素。
