纳米姜黄素在实验性艾氏腹水癌荷瘤动物中的潜在抗癌活性及其作用机制。
Potential anticancer activity and mechanism of action of nanoformulated curcumin in experimental Ehrlich ascites carcinoma-bearing animals.
机构信息
Biochemistry Division, Faculty of Science, Mansoura University, 35516, Egypt.
Zoology Department, Faculty of Science, Mansoura University, 35516, Egypt.
出版信息
Nanomedicine (Lond). 2019 Mar;14(5):553-573. doi: 10.2217/nnm-2018-0298. Epub 2019 Feb 27.
AIM
To study the potential use of nanoformulations of curcumin (CUR); CUR-loaded pluronic nanomicelles (CURnp1), and CUR-loaded poly(lactic-co-glycolic acid) nanoparticles (CURnp) as antitumor agents in Ehrlich ascites carcinoma-bearing animals, and their mechanism of action.
MATERIALS & METHODS: CURnp and CURnp were prepared, characterized and tested against Ehrlich ascites carcinoma-bearing mice. Superoxide dismutase, catalase (CAT), glutathione, malondialdehyde, histopathological, immunohistochemical studies, cell cycle and caspase-3 were investigated.
RESULTS & CONCLUSION: CURnp destroyed tumors via increasing superoxide dismutase, CAT and glutathione, decreasing malondialdehyde through inducing apoptosis by decreasing Ki-67 and Bcl2 expression and activating caspase-3 leading to inhibition of proliferation and cell cycle arrest with progression at G1/S phase. The study demonstrated for the first time superiority of CURnp over native CUR and CURnp as anticancer agents.
目的
研究姜黄素（CUR）的纳米制剂；CUR 负载的泊洛沙姆纳米胶束（CURnp1）和 CUR 负载的聚乳酸-共-羟基乙酸纳米粒（CURnp）作为抗肿瘤药物在艾氏腹水癌荷瘤动物中的潜在用途及其作用机制。
材料与方法
制备、表征 CURnp 和 CURnp，并对艾氏腹水癌荷瘤小鼠进行测试。检测超氧化物歧化酶、过氧化氢酶（CAT）、谷胱甘肽、丙二醛、组织病理学、免疫组织化学研究、细胞周期和 caspase-3。
结果与结论
CURnp 通过增加超氧化物歧化酶、CAT 和谷胱甘肽，降低丙二醛，通过降低 Ki-67 和 Bcl2 表达，激活 caspase-3 诱导细胞凋亡，从而抑制增殖和细胞周期停滞，使细胞周期进展到 G1/S 期，从而破坏肿瘤。该研究首次证明了 CURnp 优于天然 CUR 和 CURnp 作为抗癌药物。
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