Biochemistry Division, Faculty of Science, Mansoura University, 35516, Egypt.
Zoology Department, Faculty of Science, Mansoura University, 35516, Egypt.
Nanomedicine (Lond). 2019 Mar;14(5):553-573. doi: 10.2217/nnm-2018-0298. Epub 2019 Feb 27.
To study the potential use of nanoformulations of curcumin (CUR); CUR-loaded pluronic nanomicelles (CURnp1), and CUR-loaded poly(lactic-co-glycolic acid) nanoparticles (CURnp) as antitumor agents in Ehrlich ascites carcinoma-bearing animals, and their mechanism of action.
MATERIALS & METHODS: CURnp and CURnp were prepared, characterized and tested against Ehrlich ascites carcinoma-bearing mice. Superoxide dismutase, catalase (CAT), glutathione, malondialdehyde, histopathological, immunohistochemical studies, cell cycle and caspase-3 were investigated.
RESULTS & CONCLUSION: CURnp destroyed tumors via increasing superoxide dismutase, CAT and glutathione, decreasing malondialdehyde through inducing apoptosis by decreasing Ki-67 and Bcl2 expression and activating caspase-3 leading to inhibition of proliferation and cell cycle arrest with progression at G1/S phase. The study demonstrated for the first time superiority of CURnp over native CUR and CURnp as anticancer agents.
研究姜黄素（CUR）的纳米制剂；CUR 负载的泊洛沙姆纳米胶束（CURnp1）和 CUR 负载的聚乳酸-共-羟基乙酸纳米粒（CURnp）作为抗肿瘤药物在艾氏腹水癌荷瘤动物中的潜在用途及其作用机制。
制备、表征 CURnp 和 CURnp，并对艾氏腹水癌荷瘤小鼠进行测试。检测超氧化物歧化酶、过氧化氢酶（CAT）、谷胱甘肽、丙二醛、组织病理学、免疫组织化学研究、细胞周期和 caspase-3。
CURnp 通过增加超氧化物歧化酶、CAT 和谷胱甘肽，降低丙二醛，通过降低 Ki-67 和 Bcl2 表达，激活 caspase-3 诱导细胞凋亡，从而抑制增殖和细胞周期停滞，使细胞周期进展到 G1/S 期，从而破坏肿瘤。该研究首次证明了 CURnp 优于天然 CUR 和 CURnp 作为抗癌药物。
