用于治疗链脲佐菌素诱导糖尿病的纳米制剂天然疗法：姜黄素纳米制剂的潜在用途
Nanoformulated natural therapeutics for management of streptozotocin-induced diabetes: potential use of curcumin nanoformulation.
作者信息
El-Far Yousra M, Zakaria Mahmoud M, Gabr Mahmoud M, El Gayar Amal M, Eissa Laila A, El-Sherbiny Ibrahim M
机构信息
Department of Biochemistry, Faculty of Pharmacy, Mansoura University, 35516, Egypt.
Urology & Nephrology Center, Mansoura, 35516, Egypt.
出版信息
Nanomedicine (Lond). 2017 Jul;12(14):1689-1711. doi: 10.2217/nnm-2017-0106. Epub 2017 Jun 21.
AIM
The goal of this study was to improve curcumin (CUR) aqueous solubility and bioavailability via nanoformulation, and then study its activity and mechanism of action as an antidiabetic agent.
METHODS
CUR-loaded pluronic nanomicelles (CURnp) were prepared and characterized. Biochemical assessments were performed as well as histological, confocal and RTPCR studies on pancreatic target tissues.
RESULTS
CURnp with a diameter of 333 ± 6 nm and ζ potential of -26.1 mv were obtained. Antidiabetic action of CURnp was attributed to significant upregulation of Pdx-1 and NKx6.1 gene expression and achievement of optimum redox balance, which led to alleviation of streptozotocin-induced β-cell damage via a significant upregulation in insulin gene expression proved by RTPCR studies and by the presence of 40% insulin positive cells through confocal microscope studies on pancreatic tissue.
目的
本研究的目的是通过纳米制剂提高姜黄素（CUR）的水溶性和生物利用度，然后研究其作为抗糖尿病药物的活性和作用机制。
方法
制备并表征了负载姜黄素的普朗尼克纳米胶束（CURnp）。对胰腺靶组织进行了生化评估以及组织学、共聚焦和RTPCR研究。
结果
获得了直径为333±6nm、ζ电位为-26.1mv的CURnp。CURnp的抗糖尿病作用归因于Pdx-1和NKx6.1基因表达的显著上调以及最佳氧化还原平衡的实现，这通过RTPCR研究证明胰岛素基因表达的显著上调以及通过对胰腺组织的共聚焦显微镜研究发现40%的胰岛素阳性细胞，从而减轻了链脲佐菌素诱导的β细胞损伤。
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