明尼苏达系统角膜保存法。

Lindstrom R L, Doughman D J, Skelnik D L, Mindrup E A

Br J Ophthalmol. 1986 Jan;70(1):47-54. doi: 10.1136/bjo.70.1.47.

The clinical and laboratory results with a modified Minnesota system of organ culture corneal preservation are presented. A refinement of our preservation technique using a closed system, as well as the addition of chondroitin sulphate to the medium is presented. Laboratory results show preservation of corneal endothelial integrity for at least 21 days with maintenance of normal corneal thickness. In addition, a 10-day quarantine system reduces the risk of donor contamination and secondary endophthalmitis. Preliminary results of the 34 degrees C and 4 degrees C closed Minnesota corneal preservation system using chondroitin sulphate show that it is safe and efficacious and allows intermediate to long-term maintenance of sterile thin tissue prior to corneal transplantation.

本文展示了采用改良明尼苏达器官培养角膜保存系统的临床和实验室结果。介绍了我们使用封闭系统对保存技术的改进，以及在培养基中添加硫酸软骨素的情况。实验室结果表明，角膜内皮完整性可保存至少21天，同时角膜厚度保持正常。此外，10天的检疫系统降低了供体污染和继发性眼内炎的风险。使用硫酸软骨素的34摄氏度和4摄氏度封闭明尼苏达角膜保存系统的初步结果表明，该系统安全有效，能够在角膜移植前对无菌薄组织进行中长期保存。