Department of Chemical Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, Nadia, 741246, India.
Chem Commun (Camb). 2019 Mar 12;55(22):3294-3297. doi: 10.1039/c9cc00018f.
The carbonic anhydrase (CA) enzyme reversibly transforms carbon dioxide and water to a carbonate ion and a proton. Photoresponsive enzyme mimics, where the CA-activity can be turned on and off reversibly with light, have not been reported so far. We have designed an active site mimic that offers reversible control of the catalytic activity using light. Moreover, in the presence of a cationic polymer, we have demonstrated that the CA-activity was further enhanced by stabilizing the transition state with the cis-form of the enzyme mimic which can catalyze the hydration of gaseous CO2.
碳酸酐酶（CA）酶可将二氧化碳和水可逆地转化为碳酸根离子和质子。迄今为止，还没有报道过光响应的酶模拟物，其中 CA 活性可以用光可逆地开启和关闭。我们设计了一种活性位点模拟物，可使用光对催化活性进行可逆控制。此外，在阳离子聚合物存在的情况下，我们证明通过酶模拟物的顺式形式稳定过渡态，可以进一步增强 CA 活性，从而催化气态 CO2 的水合作用。
