McCallum B D, Tekauz A, Gilbert J
Cereal Research Centre, Agriculture and Agri-Food Canada, Winnipeg, Manitoba, R3T 2M9, Canada.
Plant Dis. 2004 Feb;88(2):167-174. doi: 10.1094/PDIS.2004.88.2.167.
Fusarium head blight (FHB) is currently the primary disease of concern in barley in Canada and sources of FHB resistance need to be identified. A diverse collection of 77 two-rowed and 81 six-rowed barley lines was screened for resistance to FHB in inoculated, irrigated nurseries from 1995 to 1998. Barley spikes were spray inoculated with conidia of Fusarium graminearum and visual symptoms of FHB were scored to determine an FHB index. Deoxynivalenol (DON) content was determined from harvested seed samples during 1997 and 1998. Although there was variation in the average FHB index and DON content among the different years of testing, the rankings of the lines generally were correlated among the years of testing and the two measures of FHB. Based on the FHB index from 1995 and 1996, a subset of 18 lines with low FHB and 12 lines with high FHB were compared with 20 FHB resistance sources and 7 Canadian lines or cultivars during 1997 and 1998 in both inoculated and noninoculated nurseries. Lines Nepolegajuscij, Krasnojarskij, Nordic, Murakaki-mochi, Golozernyj 1, Maris Mink, Symko, Ussurijskij 8, Suvenir, and Canadian cultivars AC Sterling and Morrison were similar to the best resistance sources (Zhedar1, Seijo II, and Chevron) and had consistently low DON content.
小麦赤霉病(FHB)目前是加拿大大麦关注的主要病害,需要鉴定抗FHB的来源。1995年至1998年期间,在接种灌溉的苗圃中,对77个二棱大麦品系和81个六棱大麦品系的多样化群体进行了FHB抗性筛选。用禾谷镰刀菌的分生孢子对大麦穗进行喷雾接种,并对FHB的视觉症状进行评分以确定FHB指数。在1997年和1998年期间,从收获的种子样本中测定脱氧雪腐镰刀菌烯醇(DON)含量。尽管在不同测试年份中平均FHB指数和DON含量存在差异,但品系的排名在不同测试年份以及FHB的两种测量方法之间通常具有相关性。基于1995年和1996年的FHB指数,在1997年和1998年期间,在接种和未接种的苗圃中,将18个低FHB品系和12个高FHB品系的子集与20个FHB抗性来源以及7个加拿大品系或品种进行了比较。品系Nepolegajuscij、Krasnojarskij、Nordic、Murakaki-mochi、Golozernyj 1、Maris Mink、Symko、Ussurijskij 8、Suvenir以及加拿大品种AC Sterling和Morrison与最佳抗性来源(Zhedar1、Seijo II和Chevron)相似,并且DON含量一直较低。