Khanal Raja, Choo Thin Meiw, Xue Allen G, Vigier Bernard, Savard Marc E, Blackwell Barbara, Wang Junmei, Yang Jianming, Martin Richard A
Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, Ontario K1A 0C6, Canada.
Chinese Barley Improvement Centre, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
Plant Pathol J. 2021 Oct;37(5):455-464. doi: 10.5423/PPJ.OA.06.2021.0094. Epub 2021 Oct 1.
Forty-eight spring barley genotypes were evaluated for deoxynivalenol (DON) concentration under natural infection across 5 years at Harrington, Prince Edward Island. These genotypes were also evaluated for Fusarium head blight (FHB) severity and DON concentration under field nurseries with artificial inoculation of Fusarium graminearum by the grain spawn method across 2 years at Ottawa, Ontario, and one year at Hangzhou, China. Additionally, these genotypes were also evaluated for FHB severity under greenhouse conditions with artificial inoculation of F. graminearum by conidial suspension spray method across 3 years at Ottawa, Ontario. The objective of the study was to investigate if reactions of barley genotypes to artificial FHB inoculation correlate with reactions to natural FHB infection. DON concentration under natural infection was positively correlated with DON concentration (r = 0.47, P < 0.01) and FHB incidence (r = 0.56, P < 0.01) in the artificially inoculated nursery with grain spawn method. Therefore, the grain spawn method can be used to effectively screen for low DON. FHB severity, generated from greenhouse spray, however, was not correlated with DON concentration (r = 0.12, P > 0.05) under natural infection and it was not correlated with DON concentration (r = -0.23, P > 0.05) and FHB incidence (r = 0.19, P > 0.05) in the artificially inoculated nursery with grain spawn method. FHB severity, DON concentration, and yield were affected by year, genotype, and the genotype × year interaction. The effectiveness of greenhouse spray inoculation for indirect selection for low DON concentration requires further studies. Nine of the 48 genotypes were found to contain low DON under natural infection. Island barley had low DON and also had high yield.
在爱德华王子岛的哈灵顿，对48个春大麦基因型进行了为期5年的自然感染条件下脱氧雪腐镰刀菌烯醇（DON）浓度评估。这些基因型还在安大略省渥太华进行了为期2年、在中国杭州进行了为期1年的田间苗圃人工接种禾谷镰刀菌的条件下，评估了赤霉病（FHB）严重程度和DON浓度，接种方法为麦粒接种法。此外，在安大略省渥太华，对这些基因型进行了为期3年的温室条件下人工接种禾谷镰刀菌分生孢子悬浮液喷雾法的FHB严重程度评估。本研究的目的是调查大麦基因型对人工接种FHB的反应是否与对自然FHB感染的反应相关。自然感染条件下的DON浓度与麦粒接种法人工接种苗圃中的DON浓度（r = 0.47，P < 0.01）和FHB发病率（r = 0.56，P < 0.01）呈正相关。因此，麦粒接种法可用于有效筛选低DON含量的品种。然而，温室喷雾产生的FHB严重程度与自然感染条件下的DON浓度（r = 0.12，P > 0.05）无关，且与麦粒接种法人工接种苗圃中的DON浓度（r = -0.23，P > 0.05）和FHB发病率（r = 0.19，P > 0.05）无关。FHB严重程度、DON浓度和产量受年份、基因型以及基因型×年份互作的影响。温室喷雾接种用于间接选择低DON浓度的有效性需要进一步研究。在48个基因型中，有9个在自然感染条件下含有低DON。海岛大麦DON含量低且产量高。
