美洲菜豆上附着丝核菌的表型和基因型特征

Phenotypic and Genotypic Characterization of Uromyces appendiculatus from Phaseolus vulgaris in the Americas.

作者信息

Araya C M, Alleyne A T, Steadman J R, Eskridge K M, Coyne D P

机构信息

Department of Plant Pathology, University of Nebraska - Lincoln, Lincoln, NE 68583-0722.

Department of Biometry, University of Nebraska - Lincoln, Lincoln, NE 68583-0712.

出版信息

Plant Dis. 2004 Aug;88(8):830-836. doi: 10.1094/PDIS.2004.88.8.830.

DOI:10.1094/PDIS.2004.88.8.830

PMID:30812510

Abstract

Populations of 90 Uromyces appendiculatus isolates were collected from throughout the Americas and evaluated for virulence on 19 standard bean rust differentials, and also on 12 landraces of Phaseolus vulgaris from South and Central America. The landrace differentials represented geographical centers of bean domestication. Three groups were observed. Two groups were isolates from centers of bean domestication and a third heterogeneous group comprised isolates from countries in South and Central America. Molecular analysis using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was also conducted on these isolates. Cluster analysis of the molecular profiles showed three groups that corresponded to those obtained by virulence tests. These results show a clear differentiation of the pathogen population along similar lines as its host and suggest parallel evolution in the bean rust pathosystem.

摘要

从美洲各地收集了90株菜豆锈菌（Uromyces appendiculatus）分离株，并对其在19个标准菜豆锈病鉴别品种以及来自南美洲和中美洲的12个菜豆地方品种上的毒力进行了评估。这些地方品种鉴别品种代表了菜豆驯化的地理中心。观察到三个组。两组是来自菜豆驯化中心的分离株，第三组异质组包括来自南美洲和中美洲国家的分离株。还对这些分离株进行了使用随机扩增多态性DNA-聚合酶链反应（RAPD-PCR）的分子分析。分子图谱的聚类分析显示出三组，与通过毒力测试获得的组相对应。这些结果表明病原菌群体与其寄主沿着相似的路线明显分化，并表明菜豆锈病病理系统中存在平行进化。