Hurtado-Gonzales Oscar P, Valentini Giseli, Gilio Thiago A S, Martins Alexandre M, Song Qijian, Pastor-Corrales Marcial A
Soybean Genomics and Improvement Laboratory, United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center-West, Maryland 20705.
Departamento de Agronomia, Universidade Estadual de Maringá, PR 87020900, Brazil.
G3 (Bethesda). 2017 Feb 9;7(2):557-569. doi: 10.1534/g3.116.036061.
Bean rust, caused by , is a devastating disease of common bean () in the Americas and Africa. The historically important gene confers resistance to many races of the highly variable bean rust pathogen that overcome other rust resistance genes. Existing molecular markers tagging for use in marker-assisted selection produce false results. Here, we describe the fine mapping of the locus for the development of highly accurate markers linked to An F population from the cross Pinto 114 (susceptible) × Aurora (resistant with ) was evaluated for its reaction to four different races of A bulked segregant analysis using the SNP chip BARCBEAN6K_3 placed the approximate location of in the lower arm of chromosome Pv11. Specific SSR and SNP markers and haplotype analysis of 18 sequenced bean varieties positioned in a 46.5 kb genomic region from 46.96 to 47.01 Mb on Pv11. We discovered in this region the SS68 KASP marker that was tightly linked to Validation of SS68 on a panel of 130 diverse common bean cultivars containing all known rust resistance genes revealed that SS68 was highly accurate and produced no false results. The SS68 marker will be of great value in pyramiding with other rust resistance genes. It will also significantly reduce time and labor associated with the current phenotypic detection of This is the first utilization of fine mapping to discover markers linked to rust resistance in common bean.
由[病原体名称未给出]引起的菜豆锈病是美洲和非洲普通菜豆（Phaseolus vulgaris）的一种毁灭性病害。具有历史重要性的[基因名称未给出]基因赋予了对高度可变的菜豆锈病病原菌许多小种的抗性，这些小种能克服其他锈病抗性基因。现有的用于标记辅助选择的标记[基因名称未给出]的分子标记会产生错误结果。在此，我们描述了[基因名称未给出]位点的精细定位，以开发与[基因名称未给出]连锁的高精度标记。对杂交组合Pinto 114（感病）×Aurora（携带[基因名称未给出]抗病）产生的F群体对四种不同菜豆锈病小种的反应进行了评估。使用SNP芯片BARCBEAN6K_3进行的混合分组分析法将[基因名称未给出]的大致位置定位在染色体Pv11的短臂上。特定的SSR和SNP标记以及对18个测序菜豆品种的单倍型分析将[基因名称未给出]定位在Pv11上46.96至47.01 Mb的46.5 kb基因组区域内。我们在该区域发现了与[基因名称未给出]紧密连锁的SS68 KASP标记。在包含所有已知锈病抗性基因的130个不同普通菜豆品种的面板上对SS68进行验证，结果表明SS68高度准确且未产生错误结果。SS68标记在将[基因名称未给出]与其他锈病抗性基因聚合方面将具有重要价值。它还将显著减少与当前[基因名称未给出]表型检测相关的时间和劳动力。这是首次利用精细定位来发现普通菜豆中与锈病抗性连锁的标记。
