Todd Antonette R, Donofrio Nicole, Sripathi Venkateswara R, McClean Phillip E, Lee Rian K, Pastor-Corrales Marcial, Kalavacharla Venu Kal
Department of Agriculture and Natural Resources, Delaware State University, Dover, DE 19901, USA.
Department of Plant and Soil Sciences, University of Delaware, Newark, DE 19716, USA.
Int J Mol Sci. 2017 May 23;18(6):1109. doi: 10.3390/ijms18061109.
Common bean ( L.) is an important legume, useful for its high protein and dietary fiber. The fungal pathogen (Pers.) Unger can cause major loss in susceptible varieties of the common bean. The locus provides race specific resistance to virulent strains or races of the bean rust pathogen along with , (Complements resistance gene), which is required for -mediated rust resistance. In this study, we inoculated two common bean genotypes (resistant "Sierra" and susceptible crg) with rust race 53 of , isolated leaf RNA at specific time points, and sequenced their transcriptomes. First, molecular markers were used to locate and identify a 250 kb deletion on chromosome 10 in mutant crg (which carries a deletion at the locus). Next, we identified differential expression of several disease resistance genes between Mock Inoculated (MI) and Inoculated (I) samples of "Sierra" leaf RNA within the 250 kb delineated region. Both marker assisted molecular profiling and RNA-seq were used to identify possible transcriptomic locations of interest regarding the resistance in the common bean to race 53. Identification of differential expression among samples in disease resistance clusters in the bean genome may elucidate significant genes underlying rust resistance. Along with preserving favorable traits in the crop, the current research may also aid in global sustainability of food stocks necessary for many populations.
普通菜豆（Phaseolus vulgaris L.）是一种重要的豆科植物，因其高蛋白和膳食纤维而具有重要价值。真菌病原体菜豆锈菌（Uromyces appendiculatus (Pers.) Unger）会使普通菜豆的易感品种遭受重大损失。该Rpg1位点提供对菜豆锈病病原体的强毒株系或生理小种的小种特异性抗性，同时还需要Rpg4（互补抗性基因）来介导对锈病的抗性。在本研究中，我们用菜豆锈菌的生理小种53接种了两种普通菜豆基因型（抗性品种“Sierra”和易感品种crg），在特定时间点分离叶片RNA，并对其转录组进行测序。首先，使用分子标记定位并鉴定了突变体crg（其Rpg1位点存在缺失）第10号染色体上的一个250 kb缺失。接下来，我们在划定的250 kb区域内，鉴定了“Sierra”叶片RNA的Mock Inoculated（MI，模拟接种）和Inoculated（I，接种）样本之间几个抗病基因的差异表达。标记辅助分子分析和RNA测序都被用于确定普通菜豆对生理小种53抗性相关的可能感兴趣的转录组位置。鉴定菜豆基因组中抗病基因簇样本间的差异表达，可能会阐明锈病抗性的重要基因。除了保留作物中的优良性状外，当前的研究还可能有助于许多人群所需粮食储备的全球可持续性。
