Vakalounakis D J, Wang Z, Fragkiadakis G A, Skaracis G N, Li D-B
National Agricultural Research Foundation (N.AG.RE.F.), Plant Protection Institute, P.O. Box 2228, 710 03 Heraklio, Crete, Greece.
Zhejiang University, College of Agriculture and Biotechnology, Biotechnology Institute, 268 Kaixuan Rd., Hangzhou 310029, China.
Plant Dis. 2004 Jun;88(6):645-649. doi: 10.1094/PDIS.2004.88.6.645.
Thirty-four isolates of Fusarium oxysporum, obtained in China from cucumber plants showing either Fusarium wilt (F. oxysporum f. sp. cucumerinum) or root and stem rot (F. oxysporum f. sp. radicis-cucumerinum) symptoms, were characterized by pathogenicity, vegetative compatibility, and random amplified polymorphic DNA (RAPD). Of these, 23 isolates were identified by pathogenicity as F. oxysporum f. sp. cucumerinum, and one as F. oxysporum f. sp. radicis-cucumerinum, while 10 isolates were avirulent on cucumber, melon, sponge gourd, and pumpkin. The Chinese isolates of F. oxysporum f. sp. cucumerinum were assigned to RAPD groups III and XXI and to vegetative compatibility group (VCG) 0183, four new VCGs, 0184 to 0187, and a single-member VCG included in the artificial VCG 018-. The Chinese isolate of F. oxysporum f. sp. radicis-cucumerinum was assigned to RAPD group I and bridging VCG 0260/0261. The occurrence of F. oxysporum f. sp. radicis-cucumerinum on cucumber is reported for the first time in China.
从中国表现出枯萎病（尖孢镰刀菌黄瓜专化型）或根腐和茎腐病（尖孢镰刀菌黄瓜根腐专化型）症状的黄瓜植株上获得了34株尖孢镰刀菌分离株，通过致病性、营养体亲和性和随机扩增多态性DNA（RAPD）对其进行了鉴定。其中，23株分离株经致病性鉴定为尖孢镰刀菌黄瓜专化型，1株为尖孢镰刀菌黄瓜根腐专化型，而10株分离株对黄瓜、甜瓜、丝瓜和南瓜无致病性。中国的尖孢镰刀菌黄瓜专化型分离株被归入RAPD组III和XXI以及营养体亲和群（VCG）0183、四个新的VCG（0184至0187）以及人工VCG 018 -中的一个单成员VCG。中国的尖孢镰刀菌黄瓜根腐专化型分离株被归入RAPD组I和桥接VCG 0260/0261。尖孢镰刀菌黄瓜根腐专化型在中国黄瓜上的发生情况首次被报道。
