Nita M, Ellis M A, Madden L V
Department of Plant Pathology, The Ohio State University, Wooster, 44691.
Plant Dis. 2003 May;87(5):579-584. doi: 10.1094/PDIS.2003.87.5.579.
Temperature, leaf wetness, and leaflet age effects on infection of strawberry foliage by Phomopsis obscurans were examined in controlled-environment experiments. A mid-season ('Honeoye') and early-season ('Earliglow') cultivar were used. Tested temperatures were 10, 15, 20, 25, 30, and 35°C, and tested wetness periods were 5, 10, 15, 20, 25, and 35 h. Leaflets were labeled based on age: 0 to 1, 2 to 6, and 7 to 14 days old. Effects of temperature, wetness duration, and leaflet age on the logit of disease incidence and severity were quantified using a linear mixed model analysis of variance (ANOVA). Age, wetness duration, and their interaction significantly affected these measures of disease. Disease intensity decreased dramatically with increasing leaflet age at the time of infection, indicating a decrease in susceptibility with maturation of foliage, and increased with increasing wetness duration. Temperature only affected disease incidence with 'Honeoye'. A prediction model was developed for leaflet infection based on ANOVA results. Coefficients of determination were approximately 0.8 for both cultivars and measures of disease (incidence and severity), indicating that disease could be described accurately based on environmental conditions and leaflet age.
在控制环境实验中，研究了温度、叶片湿度和小叶年龄对草莓叶片被隐匿拟茎点霉感染的影响。使用了一个中季品种（‘哈尼耶’）和一个早季品种（‘早熟’）。测试温度为10、15、20、25、30和35°C，测试湿度持续时间为5、10、15、20、25和35小时。根据小叶年龄进行标记：0至1天、2至6天和7至14天。使用线性混合模型方差分析（ANOVA）量化温度、湿度持续时间和小叶年龄对疾病发病率和严重程度对数的影响。年龄、湿度持续时间及其相互作用对这些疾病指标有显著影响。在感染时，随着小叶年龄的增加，疾病强度显著降低，表明随着叶片成熟易感性降低，且随着湿度持续时间的增加而增加。仅温度对‘哈尼耶’品种的疾病发病率有影响。基于方差分析结果建立了小叶感染预测模型。两个品种以及疾病指标（发病率和严重程度）的决定系数约为0.8，表明可以根据环境条件和小叶年龄准确描述疾病情况。
