Nanobioengineering Division, Nanotechnology Department, School of Advanced Technologies, Iran University of Science and Technology (IUST), Tehran, Iran.
Nanobioengineering Division, Nanotechnology Department, School of Advanced Technologies, Iran University of Science and Technology (IUST), Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2019 May;98:1087-1096. doi: 10.1016/j.msec.2018.12.140. Epub 2019 Jan 9.
In this research, novel monticellite/hydroxyapatite (HA) ceramic composites were successfully prepared by mechanical method. The ability of nanostructured monticellite-based ceramic composites to form a suitable bond to living hard tissues, and stimulate osteoblast-like cells proliferation may be different for various ratios of the reinforcement to monticellite matrix. The differences in physico-chemical characteristics, bone-like apatite formation, cytocompatibility, cell viability and in vitro osteogenic activity of nanostructured monticellite/HA ceramic composites were explored. The surface reactivity and bioactivity of the composite samples were evaluated in vitro in simulated body fluid (SBF). A comparative time- and dose-dependent MTT test showed that the ions release from nanostructured monticellite/HA composites dissolution significantly stimulated cell proliferation and growth than control at a certain concentration range. The cells viability exposed to the composite extract was higher than control and mineral material of bone (HA), illustrating that cytocompatibility was improved due to the presence of magnesium (Mg) and silicon (Si) elements in the composite structure. The comparative results of alkaline phosphatase (ALP) bioactivity assay showed that the osteogenic proliferation of osteoblast-like G292 cell was increased more by the ceramic composites extract than control. These comparative results demonstrated that nanostructured monticellite-based ceramic composites possessed good in vitro bioactivity, cytocompatibility and osteogenic properties, and may be utilized as the promising bioactive materials for bone tissue regeneration and replacement.
在这项研究中，通过机械方法成功制备了新型镁霞石/羟基磷灰石（HA）陶瓷复合材料。基于纳米结构镁霞石的陶瓷复合材料与活体硬组织形成合适结合并刺激成骨样细胞增殖的能力可能因增强剂与镁霞石基质的不同比例而有所不同。研究了纳米结构镁霞石/HA 陶瓷复合材料的物理化学特性、类骨磷灰石形成、细胞相容性、细胞活力和体外成骨活性的差异。在模拟体液（SBF）中体外评估了复合样品的表面反应性和生物活性。比较时间和剂量依赖性 MTT 试验表明，在一定浓度范围内，纳米结构镁霞石/HA 复合材料溶解释放的离子显著刺激细胞增殖和生长，比对照物高。暴露于复合材料浸提液的细胞活力高于对照物和骨（HA）的矿物材料，这表明由于复合材料结构中存在镁（Mg）和硅（Si）元素，细胞相容性得到了提高。碱性磷酸酶（ALP）生物活性测定的比较结果表明，陶瓷复合材料浸提液对成骨样 G292 细胞的成骨增殖的促进作用大于对照物。这些比较结果表明，基于纳米结构镁霞石的陶瓷复合材料具有良好的体外生物活性、细胞相容性和成骨性能，可能可用作骨组织再生和替代的有前途的生物活性材料。
