Shubnikov Institute of Crystallography, Russian Academy of Sciences, 59 Leninskii pr., Moscow, 119333, Russia; Department of Nano- and Biomedical Technologies, Saratov State University, 83 Astrakhanskaya st., 410012 Saratov, Russia; Department of Molecular Biotechnology, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Department of Nano- and Biomedical Technologies, Saratov State University, 83 Astrakhanskaya st., 410012 Saratov, Russia.
Mater Sci Eng C Mater Biol Appl. 2019 May;98:1114-1121. doi: 10.1016/j.msec.2019.01.030. Epub 2019 Jan 9.
A novel type of microcontainers based on hollow silver alginate microspheres and magnetite nanoparticles is reported as development of recently published technology. Magnetite nanoparticles were incorporated by two methods - co-precipitation with porous calcium carbonate during template formation and adsorption onto CaCO particles or microcontainers' shell. Amount of magnetite loaded and microshells size (4.6 to 6.9 μm) were found to depend on the chosen method for magnetite nanoparticles incorporation. Stability of hollow microshells in saline, phosphate buffer and culturing media was studied. Microcontainers' susceptibility to magnetic field was investigated in solutions of varied viscosity, and their group movement velocity under constant magnetic field was evaluated by sequential optical microscopy imaging. Cell viability tests with prepared microshells were performed that demonstrated negligible cytotoxicity effect on human dermal fibroblasts cells. With HeLa cells moderate viability inhibition was found at high carriers:cells ratio at early time points which is attributed to more active and receptor-mediated endocytosis of carriers as well as known cytotoxicity of magnetite in some cancer cells. At 24 and 48 h time points HeLa cells proliferation fully recovers. Reported data opens perspectives for further biomedical-oriented studies and application of this novel kind of microcontainers with a number of techniques applicable for imaging, control and triggered cargo release provided by presence of silver and magnetite nanoparticles in the carriers and their suitability for further versatile functionalization by traditional LbL approach if needed.
本文报道了一种新型的微容器，它基于中空的海藻酸钠微球和磁铁矿纳米粒子。这种微容器是在最近发表的技术基础上开发的。磁铁矿纳米粒子通过两种方法掺入 - 在模板形成过程中与多孔碳酸钙共沉淀，以及吸附到 CaCO 颗粒或微容器壳上。负载的磁铁矿量和微壳的尺寸（4.6 至 6.9 μm）取决于所选择的掺入磁铁矿纳米粒子的方法。研究了中空微壳在盐水中、磷酸盐缓冲液和培养基中的稳定性。在不同粘度的溶液中研究了微容器对磁场的敏感性，并通过连续光学显微镜成像评估了它们在恒定磁场下的集体运动速度。对制备的微壳进行了细胞活力测试，结果表明对人皮肤成纤维细胞几乎没有细胞毒性作用。对于 HeLa 细胞，在高载体：细胞比的早期时间点发现了中等的活力抑制，这归因于载体更活跃和受体介导的内吞作用，以及磁铁矿在一些癌细胞中的已知细胞毒性。在 24 和 48 小时时间点，HeLa 细胞的增殖完全恢复。报道的数据为进一步的生物医学导向研究和这种新型微容器的应用开辟了前景，这种微容器具有多种技术，可用于成像、控制和触发货物释放，这是由于载体中存在银和磁铁矿纳米粒子以及它们适合进一步通过传统的 LbL 方法进行多功能化。
