NanoBioMedical Centre, Adam Mickiewicz University, Poznań, Poland.
Nanotechnology. 2017 Feb 3;28(5):055603. doi: 10.1088/1361-6528/28/5/055603. Epub 2016 Dec 28.
Magnetite nanoparticles (NPs) decorated with silver (magnetite/Ag) are intensively investigated due to their application in the biomedical field. We demonstrate that the increase of silver content on the surface of nanoparticles improves the adsorptivity of antibiotic rifampicin as well as antibacterial properties. The use of ginger extract allowed to improve the silver nucleation on the magnetite surface that resulted in an increase of silver content. Physicochemical and functional characterization of magnetite/Ag NPs was performed. Our results show that 5%-10% of silver content in magnetite/Ag NPs is already sufficient for antimicrobial properties against Streptococcus salivarius and Staphylococcus aureus. The rifampicin molecules on the magnetite/Ag NPs surface made the spectrum of antimicrobial activity wider. Cytotoxicity evaluation of the magnetite/Ag/rifampicin NPs showed no harmful action towards normal human fibroblasts, whereas the effect on human embryonic kidney cell viability was time and dose dependent.
由于在生物医学领域的应用，表面修饰银的磁铁矿纳米颗粒（NPs）受到了广泛的关注。我们证明了纳米颗粒表面银含量的增加提高了抗生素利福平的吸附能力和抗菌性能。使用生姜提取物可以促进磁铁矿表面的银成核，从而增加银的含量。对磁铁矿/Ag NPs 的物理化学和功能特性进行了表征。结果表明，5%-10%的银含量已足以赋予磁铁矿/Ag NPs 对抗唾液链球菌和金黄色葡萄球菌的抗菌性能。利福平分子在磁铁矿/Ag NPs 表面使抗菌活性谱更宽。对磁铁矿/Ag/利福平 NPs 的细胞毒性评估表明，其对正常人类成纤维细胞没有有害作用，而对人胚肾细胞活力的影响则与时间和剂量有关。
