Center for Bioelectronics, Biosensors and Biochips (C3B®), Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA; Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA.
Department of Chemical Engineering, West Virginia University, Morgantown, WV 26506, USA.
Mater Sci Eng C Mater Biol Appl. 2019 May;98:89-100. doi: 10.1016/j.msec.2018.12.083. Epub 2018 Dec 31.
Hydrogel membranes of in-dwelling pH-responsive devices are of interest for the development of biomedical sensors that must measure small changes in pH associated with tissue acidosis. Poly(2-hydroxyethyl methacrylate)-based hydrogels possessing minor amounts of the cationogenic N-(2-aminoethyl) methacrylamide (AEMA) (4 mol%) or a tertiary amine moiety, N,N-(2-dimethylamino)ethyl methacrylamide (DMAEMA) (4 mol%) or AEMA-DMAEMA (2 mol% each) were UV cross-linked with 1 mol% tetra(ethylene glycol) diacrylate (TEGDA) and the degree of hydration, free and bound water distribution, glass transition temperature, elastic modulus, membrane resistance and protein adsorption were studied. Correlation analysis reveals that each of these biotechnical properties is strongly anti-correlated with total hydration (-0.92) and that the bound water content dominates this anti-correlation (-0.83). However, free water shows a direct, though only weak correlation with these properties (+0.5). Thus, minor changes in the hydrogel composition (~4 mol%) can significantly influence biomaterials properties and may be useful in tailoring hydrogel properties for application in biosensors and engineered tissue scaffolds.
留置 pH 响应装置的水凝胶膜对于开发生物医学传感器很有意义，因为这些传感器必须测量与组织酸中毒相关的 pH 微小变化。基于聚(2-羟乙基甲基丙烯酸酯)的水凝胶中含有少量的阳离子型 N-(2-氨基乙基)甲基丙烯酰胺（AEMA）（4 mol%）或叔胺部分 N,N-(2-二甲氨基)乙基甲基丙烯酰胺（DMAEMA）（4 mol%）或 AEMA-DMAEMA（各 2 mol%），用 1 mol%四甘醇二丙烯酸酯（TEGDA）进行了 UV 交联，并研究了水合度、游离水和结合水分布、玻璃化转变温度、弹性模量、膜电阻和蛋白质吸附。相关分析表明，这些生物技术特性中的每一个都与总水合度呈强烈的负相关（-0.92），并且结合水含量主导了这种负相关（-0.83）。然而，自由水与这些特性呈直接关系，尽管只是弱相关（+0.5）。因此，水凝胶组成的微小变化（~4 mol%）可以显著影响生物材料的性能，并且可能有助于调整水凝胶的特性，以应用于生物传感器和工程组织支架。
