Norman R L, Rivier J, Vale W, Spies H G
Fertil Steril. 1986 Feb;45(2):288-91.
Six adult ovariectomized rhesus macaques were given a gonadotropin-releasing hormone (GnRH) antagonist [Ac-beta-(2)DNAL,pFDPhe,DTrp,DArg]-GnRH, by intravenous infusion for 3 to 3.5 days to determine whether the positive feedback action of estradiol benzoate (E2B) on pituitary luteinizing hormone (LH) secretion could be inhibited by blockage of GnRH binding to pituitary gonadotropes. The LH release was suppressed when the antagonist was given either as a bolus injection every 6 hours or as a constant infusion, beginning 24 hours after the E2B was administered. Both LH release and follicle-stimulating hormone release were suppressed if the GnRH antagonist infusion began when the E2B was administered. The results suggest that continued hypothalamic GnRH stimulation of the pituitary is necessary for the full expression of the preovulatory-like gonadotropin surge that occurs in ovariectomized macaques in response to E2.
六只成年去卵巢恒河猴通过静脉输注促性腺激素释放激素（GnRH）拮抗剂[Ac-β-(2)DNAL,pFDPhe,DTrp,DArg]-GnRH 3至3.5天，以确定苯甲酸雌二醇（E2B）对垂体促黄体生成素（LH）分泌的正反馈作用是否可通过阻断GnRH与垂体促性腺激素细胞的结合而被抑制。当拮抗剂在给予E2B 24小时后，每6小时静脉推注一次或持续输注时，LH释放受到抑制。如果在给予E2B时开始输注GnRH拮抗剂，则LH释放和促卵泡激素释放均受到抑制。结果表明，下丘脑持续的GnRH刺激对于去卵巢猕猴对E2产生的类似排卵前促性腺激素激增的充分表达是必要的。
