Flamigni F, Guarnieri C, Caldarera C M
Gen Pharmacol. 1986;17(1):31-6. doi: 10.1016/0306-3623(86)90007-8.
Heart ornithine decarboxylase (ODC) from isoproterenol treated rats was compared to heart ODC from control rats. Isoproterenol administration did not significantly change Km for ornithine, but it induced a marked increase of Vmax, X Km for pyridoxal phosphate (PLP) was somewhat reduced. Both Kornm and Vmax were a function of the dithiothreitol (DTT) concentration, in a similar way for control and stimulated enzyme. Two ODC forms were detected by ion exchange chromatography in both control and isoproterenol treated hearts. In control heart, ODC specific activity was high in cytosol and nucleoli. Isoproterenol administration induced a remarkable increase of the cytosolic enzyme only.
将异丙肾上腺素处理的大鼠心脏中的鸟氨酸脱羧酶（ODC）与对照大鼠心脏中的ODC进行了比较。给予异丙肾上腺素并没有显著改变鸟氨酸的Km值，但它诱导了Vmax的显著增加，吡哆醛磷酸（PLP）的Km值有所降低。对于对照酶和受刺激的酶，Kornm和Vmax都是二硫苏糖醇（DTT）浓度的函数，方式相似。通过离子交换色谱法在对照和异丙肾上腺素处理的心脏中均检测到两种ODC形式。在对照心脏中，ODC的比活性在细胞质和核仁中较高。给予异丙肾上腺素仅诱导细胞质酶显著增加。
