Bartholeyns J
Life Sci. 1983 Mar 21;32(12):1305-12. doi: 10.1016/0024-3205(83)90804-4.
The subcellular localisation of ornithine decarboxylase and of its synthetic irreversible inhibitor, alpha-difluoromethylornithine, was investigated in control rat livers and in livers of animals in which the enzyme was induced by partial hepatectomy or by treatment with dexamethasone. Ornithine decarboxylase activity was distributed in normal rat liver between the nuclear (40%, mainly nucleolar) and the cytosolic (43%) fractions. Cytosolic liver ornithine decarboxylase was markedly induced after partial hepatectomy or treatment with dexamethasone, whereas the enzyme associated with the nuclear fraction was not induced by these procedures. The irreversible inhibitor was found only in the cytosol fraction and was totally absent from the nuclear fraction.
在对照大鼠肝脏以及经部分肝切除或地塞米松处理诱导该酶的动物肝脏中，研究了鸟氨酸脱羧酶及其合成的不可逆抑制剂α-二氟甲基鸟氨酸的亚细胞定位。鸟氨酸脱羧酶活性在正常大鼠肝脏中分布于细胞核（40%，主要是核仁）和胞质（43%）部分之间。部分肝切除或地塞米松处理后，胞质中的肝脏鸟氨酸脱羧酶明显被诱导，而与细胞核部分相关的酶未被这些操作诱导。不可逆抑制剂仅在胞质部分中发现，细胞核部分完全没有。
