单核细胞细胞毒性因子在单核细胞介导的肿瘤细胞裂解中的作用。
The role of monocyte cytotoxic factor in monocyte-mediated lysis of tumour cells.
作者信息
Espevik T, Kildahl-Andersen O, Nissen-Meyer J
出版信息
Immunology. 1986 Feb;57(2):255-9.
The role of a monocyte cytotoxic factor (CF) in monocyte-mediated lysis of leukaemia cells (K562) has been investigated using a polyclonal rabbit antiserum raised against purified CF. The CF antiserum inhibited K562 cell lysis mediated by interferon gamma (IFN-gamma)-activated monocytes. CF antiserum also inhibited monocyte-mediated lysis of antibody-coated K562 cells (AbK562). Preimmune serum at the same concentration as CF antiserum did not affect monocyte-mediated lysis, and the CF antiserum did not inhibit binding between effector and target cells, indicating that inhibition of monocyte-mediated lysis by CF antiserum was not merely a result of toxic components present in the rabbit serum, or a result of a decrease in monocyte-target cell binding. Taken together, the data suggest that CF is involved in monocyte-mediated lysis of uncoated as well as antibody-coated K562 cells.
利用针对纯化单核细胞细胞毒性因子（CF）制备的多克隆兔抗血清，研究了单核细胞细胞毒性因子（CF）在单核细胞介导的白血病细胞（K562）裂解中的作用。CF抗血清抑制了由干扰素γ（IFN-γ）激活的单核细胞介导的K562细胞裂解。CF抗血清也抑制了单核细胞介导的抗体包被的K562细胞（AbK562）的裂解。与CF抗血清浓度相同的免疫前血清不影响单核细胞介导的裂解，并且CF抗血清不抑制效应细胞与靶细胞之间的结合，这表明CF抗血清对单核细胞介导的裂解的抑制不仅仅是兔血清中存在的毒性成分的结果，也不是单核细胞-靶细胞结合减少的结果。综上所述，数据表明CF参与了单核细胞介导的未包被以及抗体包被的K562细胞的裂解。
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