单核细胞细胞毒性因子（CF）在由γ-干扰素激活的单核细胞介导的细胞生长抑制中的作用。
The role of monocyte cytotoxic factor (CF) in cytostasis mediated by IFN-gamma-activated monocytes.
作者信息
Kildahl-Andersen O, Nissen-Meyer J
出版信息
Immunology. 1985 Oct;56(2):367-72.
The role of monocyte cytotoxic factor (CF) in cytostasis mediated by lymphokine- and interferon gamma (IFN-gamma)-activated monocytes was investigated. Recombinant IFN-gamma as well as lymphokines enhanced monocyte-mediated cytostasis concomitantly with an increase in monocyte CF content. The incubation of lymphokines with monoclonal antibody against human IFN-gamma inhibited cytostasis mediated by lymphokine-exposed monocytes, indicating that the main monocyte-activating factor in the lymphokine supernatant was IFN-gamma. A neutralizing antiserum raised against purified CF inhibited monocyte-mediated cytostasis in a dose-dependent manner. When CF antiserum was added to monocytes previously exposed to lymphokines treated with a monoclonal antibody against IFN-gamma, all cytostatic activity was completely abrogated. No inhibitory effect on cytostasis was observed with preimmune serum. The results indicate that CF is an effector molecule in cytostasis mediated by lymphokine- or IFN-gamma-activated monocytes.
研究了单核细胞细胞毒性因子（CF）在淋巴因子和干扰素γ（IFN-γ）激活的单核细胞介导的细胞生长抑制中的作用。重组IFN-γ以及淋巴因子增强了单核细胞介导的细胞生长抑制，同时单核细胞CF含量增加。淋巴因子与抗人IFN-γ单克隆抗体孵育可抑制淋巴因子处理的单核细胞介导的细胞生长抑制，表明淋巴因子上清液中主要的单核细胞激活因子是IFN-γ。针对纯化的CF产生的中和抗血清以剂量依赖的方式抑制单核细胞介导的细胞生长抑制。当将CF抗血清添加到先前暴露于用抗IFN-γ单克隆抗体处理的淋巴因子的单核细胞中时，所有的细胞生长抑制活性完全消除。用免疫前血清未观察到对细胞生长抑制的抑制作用。结果表明，CF是淋巴因子或IFN-γ激活的单核细胞介导的细胞生长抑制中的效应分子。
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