Meekhanon Nattakan, Kaewmongkol Sarawan, Jirawattanapong Pichai, Kaminsonsakul Tanyanant, Kongsoi Siriporn, Chumsing Suksan, Okura Masatoshi, Ueno Yuichi, Sekizaki Tsutomu, Takamatsu Daisuke
Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Department of Farm Resources and Production Medicines, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand.
J Vet Med Sci. 2019 Apr 16;81(4):567-572. doi: 10.1292/jvms.18-0678. Epub 2019 Feb 27.
In this study, 22 bacterial isolates from swine necropsy specimens, which were biochemically identified as Streptococcus suis and other Streptococcus species, were re-examined using species-specific PCR for authentic S. suis and 16S rRNA gene sequencing for the verification of the former judge. Identification of S. suis on the basis of biochemical characteristics showed high false-positive (70.6%) and false-negative (60%) rates. The authentic S. suis showed various capsular polysaccharide synthesis gene types, including type 2 that often isolated from human cases. Five of 22 isolates did not even belong to the genus Streptococcus. These results suggested that the misidentification of the causative pathogen in routine veterinary diagnosis could be a substantial obstacle for the control of emerging infectious diseases.
在本研究中，从猪尸检标本中分离出22株细菌，经生化鉴定为猪链球菌和其他链球菌属菌种，使用针对真性猪链球菌的种特异性PCR和16S rRNA基因测序对先前的鉴定结果进行重新检测，以验证先前的判断。基于生化特征对猪链球菌进行鉴定显示出较高的假阳性率（70.6%）和假阴性率（60%）。真性猪链球菌表现出多种荚膜多糖合成基因类型，包括经常从人类病例中分离出的2型。22株分离株中有5株甚至不属于链球菌属。这些结果表明，常规兽医诊断中致病病原体的错误鉴定可能成为控制新发传染病的重大障碍。
