Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale G. Fanin 46, 40127, Bologna, Italy.
Sci Rep. 2022 Jan 31;12(1):1604. doi: 10.1038/s41598-022-05707-9.
The study aimed to assess how the post-weaning condition changes piglet peripheral blood (PB) and jejunal Peyer's patches (JPPs) as compared to the suckling period, and how these changes are associated with intestinal microbiota evolution. Sixteen pigs were slaughtered and sampled for PB, JPPs and jejunal content (JC) at weaning (26 days) or at 12 days fed on a pre-starter diet. The PB and JPP transcriptomes were analysed using mRNA-seq. The Gene Set Enrichment Analysis was used to demonstrate enriched gene clusters, depending on sampling time. Jejunal microbiota was profiled using 16S rRNA gene sequencing. Post-weaning JPPs were enriched for processes related to the activation of IFN-γ and major histocompatibility complex (MHC) class I antigen processing which clustered with the reduced abundance of the Weisella genus and Faecalibacterium prausnitzii in JC. The post-weaning microbiome differed from that seen in just-weaned pigs. For just-weaned PB, the enrichment of genes related to hemoglobin and the iron metabolism indicated the greater presence of reticulocytes and immature erythrocytes. The JPP genes involved in the I MHC and IFN-γ activations were markers of the post-weaning phase. Several genes attributable to reticulocyte and erythrocyte maturation could be interesting for testing the iron nutrition of piglets.
本研究旨在评估与哺乳期相比，断乳后仔猪外周血 (PB) 和空肠派尔集合淋巴结 (JPP) 的状况如何变化，以及这些变化如何与肠道微生物群的演变相关。16 头仔猪在断乳（26 天）或 12 天龄时接受预开食日粮喂养时被屠宰并采集 PB、JPP 和空肠内容物 (JC) 进行采样。使用 mRNA-seq 分析 PB 和 JPP 的转录组。使用基因集富集分析来展示依赖于采样时间的富集基因簇。使用 16S rRNA 基因测序来分析空肠微生物群。与 JUST 断奶仔猪相比，JPP 中的基因与 IFN-γ 和主要组织相容性复合体 (MHC) 类 I 抗原加工的激活有关，这与 JC 中魏斯氏菌属和普拉氏梭菌属丰度降低有关。与 JUST 断奶仔猪相比，JPP 中的基因与 IFN-γ 和主要组织相容性复合体 (MHC) 类 I 抗原加工的激活有关，这与 JC 中魏斯氏菌属和普拉氏梭菌属丰度降低有关。与 JUST 断奶仔猪相比，JPP 中的基因与 IFN-γ 和主要组织相容性复合体 (MHC) 类 I 抗原加工的激活有关，这与 JC 中魏斯氏菌属和普拉氏梭菌属丰度降低有关。与 JUST 断奶仔猪相比，JPP 中的基因与 IFN-γ 和主要组织相容性复合体 (MHC) 类 I 抗原加工的激活有关，这与 JC 中魏斯氏菌属和普拉氏梭菌属丰度降低有关。刚刚断奶的 PB 中与血红蛋白和铁代谢相关的基因富集表明，网织红细胞和未成熟红细胞的存在更多。参与 I MHC 和 IFN-γ 激活的 JPP 基因是断乳后阶段的标志物。一些与网织红细胞和红细胞成熟相关的基因可能对仔猪铁营养的测试很有意义。
