Molecular identification and genotyping of isolated from burn patients by PCR and ERIC-PCR.

BACKGROUND

is one of the most important agents of hospital infections. Rapid and accurate identification and genotyping of is very important, especially in burn hospitals in order to prevent the spread of related nosocomial infections and to further epidemiological studies.

MATERIAL AND METHODS

For two months, 82 isolates were collected from burn wound swabs of patients in a major burn hospital in Tehran. isolates were identified by conventional microbiological test and polymerase chain reaction (PCR) using the primers of gene, while the genetic linkage of isolates was investigated by enterobacterial repetitive intragenic consensus (ERIC)-PCR technique. Similarity, a cut-off of ⩾ 95% was considered for classifying the genotypes.

RESULTS

The molecular test (PCR) confirmed 97.56% of phenotypic results for the detection of isolates. ERIC-PCR results revealed 14 different ERIC patterns (ERIC-types) including 11 common types and three unique types.

CONCLUSION

Our findings show that we can simply and quickly detect isolates by PCR using genes and genetic diversity by ERIC-PCR, respectively. These rapid and simple techniques for the routine screening and identification of clinical isolates could be useful with epidemic potential.