Falah Faezeh, Shokoohizadeh Leili, Adabi Maryam
Department of Biology, Tonekabon Branch, Islamic Azad University, Iran.
Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Scars Burn Heal. 2019 Feb 19;5:2059513119831369. doi: 10.1177/2059513119831369. eCollection 2019 Jan-Dec.
is one of the most important agents of hospital infections. Rapid and accurate identification and genotyping of is very important, especially in burn hospitals in order to prevent the spread of related nosocomial infections and to further epidemiological studies.
For two months, 82 isolates were collected from burn wound swabs of patients in a major burn hospital in Tehran. isolates were identified by conventional microbiological test and polymerase chain reaction (PCR) using the primers of gene, while the genetic linkage of isolates was investigated by enterobacterial repetitive intragenic consensus (ERIC)-PCR technique. Similarity, a cut-off of ⩾ 95% was considered for classifying the genotypes.
The molecular test (PCR) confirmed 97.56% of phenotypic results for the detection of isolates. ERIC-PCR results revealed 14 different ERIC patterns (ERIC-types) including 11 common types and three unique types.
Our findings show that we can simply and quickly detect isolates by PCR using genes and genetic diversity by ERIC-PCR, respectively. These rapid and simple techniques for the routine screening and identification of clinical isolates could be useful with epidemic potential.
[细菌名称]是医院感染最重要的病原体之一。对[细菌名称]进行快速准确的鉴定和基因分型非常重要，尤其是在烧伤医院，以防止相关医院感染的传播并推进流行病学研究。
在两个月的时间里，从德黑兰一家大型烧伤医院的患者烧伤创面拭子中收集了82株[细菌名称]分离株。通过传统微生物学检测和使用[细菌名称]基因引物的聚合酶链反应（PCR）对[细菌名称]分离株进行鉴定，同时采用肠杆菌基因间重复共有序列（ERIC）-PCR技术研究[细菌名称]分离株的遗传连锁关系。相似性方面，将⩾95%的截断值用于基因型分类。
分子检测（PCR）对[细菌名称]分离株检测的表型结果确认率为97.56%。ERIC-PCR结果显示了14种不同的ERIC模式（ERIC型），包括11种常见类型和3种独特类型。
我们的研究结果表明，我们可以分别通过使用[细菌名称]基因的PCR简单快速地检测[细菌名称]分离株，并通过ERIC-PCR检测其遗传多样性。这些用于临床[细菌名称]分离株常规筛选和鉴定的快速简单技术可能对具有流行潜力的情况有用。
