Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Department of Aerospace & Subaquatic Medicine, Infectious Diseases & Tropical Medicine Research Center, AJA University of Medical Sciences, Tehran, Iran.
Future Microbiol. 2022 Aug;17:917-930. doi: 10.2217/fmb-2021-0225. Epub 2022 Jul 14.
To control the spread of in hospitals, it is necessary to identify the reservoir of organisms and the way they are transmitted. This study analyzed samples by BOX-PCR and enterobacterial repetitive intergenic consensus PCR techniques. Isolated strains were identified using the Microgen kit and gene. The genetic diversity of strains that were sensitive or resistant to colistin was evaluated by BOX-PCR and enterobacterial repetitive intergenic consensus PCR methods. A total of 13% of the isolates were resistant to colistin, whereas 87% of the strains were sensitive to this medication. strains that were resistant or sensitive to colistin were divided into five groups using the BOX-PCR method and six groups using the enterobacterial repetitive intergenic consensus PCR method. Rapid identification and the use of appropriate tools to control colistin-resistant clones are essential to prevent the further spread of .
为了控制医院内的传播，有必要确定病原体的储存库及其传播方式。本研究通过 BOX-PCR 和肠杆菌基因间重复一致序列 PCR 技术分析了样本。使用 Microgen 试剂盒和基因鉴定分离株。通过 BOX-PCR 和肠杆菌基因间重复一致序列 PCR 方法评估对多粘菌素敏感或耐药的菌株的遗传多样性。共有 13%的分离株对多粘菌素耐药，而 87%的菌株对该药物敏感。使用 BOX-PCR 方法将对多粘菌素耐药或敏感的菌株分为 5 组，使用肠杆菌基因间重复一致序列 PCR 方法将其分为 6 组。快速鉴定和使用适当的工具来控制多粘菌素耐药克隆对于防止进一步传播至关重要。
