ENDIA研究中1型糖尿病孕妇独特的肠道病毒组特征
Distinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study.
作者信息
Wook Kim Ki, Allen Digby W, Briese Thomas, Couper Jennifer J, Barry Simon C, Colman Peter G, Cotterill Andrew M, Davis Elizabeth A, Giles Lynne C, Harrison Leonard C, Harris Mark, Haynes Aveni, Horton Jessica L, Isaacs Sonia R, Jain Komal, Lipkin Walter Ian, Morahan Grant, Morbey Claire, Pang Ignatius C N, Papenfuss Anthony T, Penno Megan A S, Sinnott Richard O, Soldatos Georgia, Thomson Rebecca L, Vuillermin Peter J, Wentworth John M, Wilkins Marc R, Rawlinson William D, Craig Maria E
机构信息
School of Women's and Children's Health, University of New South Wales, Sydney, Australia.
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York.
出版信息
Open Forum Infect Dis. 2019 Jan 16;6(2):ofz025. doi: 10.1093/ofid/ofz025. eCollection 2019 Feb.
BACKGROUND
The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture sequencing to characterize the gut virome of pregnant women with and without type 1 diabetes (T1D), longitudinally followed in the Environmental Determinants of Islet Autoimmunity study.
METHODS
In total, 61 pregnant women (35 with T1D and 26 without) from Australia were examined. Nucleic acid was extracted from serial fecal specimens obtained at prenatal visits, and viral genomes were sequenced by virome capture enrichment. The frequency, richness, and abundance of viruses were compared between women with and without T1D.
RESULTS
Two viruses were more prevalent in pregnant women with T1D: picobirnaviruses (odds ratio [OR], 4.2; 95% confidence interval [CI], 1.0-17.1; = .046) and tobamoviruses (OR, 3.2; 95% CI, 1.1-9.3; = .037). The abundance of 77 viruses significantly differed between the 2 maternal groups (≥2-fold difference; < .02), including 8 types present at a higher abundance in women with T1D.
CONCLUSIONS
These findings provide novel insight into the composition of the gut virome during pregnancy and demonstrate a distinct profile of viruses in women with T1D.
背景
肠道细菌在人体生理学、免疫调节和疾病发病机制中的重要性已得到充分证实。相比之下，肠道病毒组的组成和动态变化在很大程度上尚不清楚；尤其是在孕期的研究较为缺乏。我们在胰岛自身免疫环境决定因素研究中，采用综合病毒组捕获测序技术，对患有和未患有1型糖尿病（T1D）的孕妇肠道病毒组进行纵向追踪，以进行特征描述。
方法
总共对来自澳大利亚的61名孕妇（35名患有T1D，26名未患）进行了检查。从产前检查时采集的系列粪便样本中提取核酸，并通过病毒组捕获富集对病毒基因组进行测序。比较了患有和未患有T1D的女性之间病毒的频率、丰富度和丰度。
结果
两种病毒在患有T1D的孕妇中更为普遍：微小双股RNA病毒（优势比[OR]，4.2；95%置信区间[CI]，1.0 - 17.1；P = 0.046）和烟草花叶病毒（OR，3.2；95% CI，1.1 - 9.3；P = 0.037）。两组孕妇之间77种病毒的丰度存在显著差异（差异≥2倍；P < 0.02），其中8种病毒在患有T1D的女性中丰度更高。
结论
这些发现为孕期肠道病毒组的组成提供了新的见解，并证明了患有T1D的女性中病毒的独特特征。
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