Jopling Helen, Yates Allen, Burgoyne Nicholas, Hayden Katharine, Chaloner Christopher, Tetlow Lesley
Department of Clinical Biochemistry Manchester University NHS Foundation Trust Manchester UK.
Burgoyne Data Science Consultancy Manchester UK.
Endocrinol Diabetes Metab. 2018 Jul 18;1(4):e00021. doi: 10.1002/edm2.21. eCollection 2018 Oct.
Anti-Müllerian Hormone (AMH) concentration is high at birth in males, demonstrating the presence of functional testicular tissue in the prepubertal period, and acting as a useful marker in the investigation of paediatric reproductive disorders. AMH also provides a tool in the investigation of female virilization, premature ovarian failure and polycystic ovarian syndrome in childhood. Robust, assay-specific paediatric AMH reference intervals are therefore required for clinical interpretation of results. The aim of this study was to derive age-specific AMH reference intervals for males and females aged 0-18 years.
Plasma samples were obtained from patients at Royal Manchester Children's Hospital and analysed for AMH using the automated Beckman Coulter Access AMH Assay. Patients under investigation for paediatric reproductive or endocrine disorders were excluded from the study.
Seven hundred and 2 patient plasma samples (465 male, 237 female) were subject to AMH measurement, and results were analysed in order to derive continuous and discrete reference intervals for the paediatric age range.
Clear discrimination between male and female AMH results was evident in the prepubertal age range, with some overlap between the genders following pubertal onset.
We have derived age-related reference intervals for plasma AMH in the paediatric age range (0-18 years) using the automated Beckman Coulter Access AMH assay which will aid in the investigation of paediatric endocrine disorders such as disorders of sexual development.
抗苗勒管激素（AMH）在男性出生时浓度较高，表明青春期前存在功能性睾丸组织，并且在小儿生殖系统疾病的调查中是一种有用的标志物。AMH还为儿童期女性男性化、卵巢早衰和多囊卵巢综合征的调查提供了一种工具。因此，临床结果解读需要可靠的、特定检测方法的小儿AMH参考区间。本研究的目的是得出0至18岁男性和女性的年龄特异性AMH参考区间。
从皇家曼彻斯特儿童医院的患者中获取血浆样本，并使用贝克曼库尔特Access AMH自动化检测法分析AMH。正在接受小儿生殖或内分泌疾病调查的患者被排除在研究之外。
对702份患者血浆样本（465例男性，237例女性）进行AMH测量，并对结果进行分析，以得出小儿年龄范围内的连续和离散参考区间。
青春期前年龄范围内，男性和女性的AMH结果有明显差异，青春期开始后两性之间有一定重叠。
我们使用贝克曼库尔特Access AMH自动化检测法得出了小儿年龄范围（0至18岁）血浆AMH的年龄相关参考区间，这将有助于小儿内分泌疾病如性发育障碍的调查。
