阿霉素，一种来自白色链霉菌 J1074 的新型异吲哚喹酮，该菌含有氟斯塔汀生物合成基因簇。

Albumycin, a new isoindolequinone from Streptomyces albus J1074 harboring the fluostatin biosynthetic gene cluster.

机构信息

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institutions of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, 510301, Guangzhou, China.

University of Chinese Academy of Sciences, 19 Yuquan Road, 100049, Beijing, China.

出版信息

J Antibiot (Tokyo). 2019 May;72(5):311-315. doi: 10.1038/s41429-019-0161-4. Epub 2019 Feb 28.

DOI:10.1038/s41429-019-0161-4

PMID:30816348

Abstract

Heterologous expression of the fluostatin biosynthetic gene cluster from the marine-derived Micromonospora rosaria SCSIO N160 in Streptomyces albus J1074 led to the isolation of a novel isoindolequinone albumycin (1) and a known isoquinolinequinone mansouramycin A (2). The structure of 1 was elucidated on the basis of detailed 1D and 2D NMR spectroscopic analysis. Mansouramycin A (2) is active against methicillin-resistant Staphylococcus aureus ATCC 43300, with a MIC of 8 μg ml, while albumycin (1) displayed negligible antibacterial activities. This study represents another example of activation of secondary metabolites that are non-relevant to the heterologously introduced biosynthetic gene cluster in a bacterial host.

摘要

海洋来源的玫瑰小单孢菌 SCSIO N160 中的 fluostatin 生物合成基因簇在链霉菌 albus J1074 中的异源表达导致分离到一个新的异吲哚醌 albumycin（1）和一个已知的异喹啉醌mansouramycin A（2）。基于详细的 1D 和 2D NMR 光谱分析阐明了 1 的结构。mansouramycin A（2）对耐甲氧西林金黄色葡萄球菌 ATCC 43300 具有活性，MIC 为 8μg/ml，而 albumycin（1）显示出几乎没有抗菌活性。本研究代表了在细菌宿主中异源引入的生物合成基因簇不相关的次级代谢物的另一个激活实例。