Institute of Biochemistry, Graz University of Technology, Graz, Austria.
J Sep Sci. 2019 May;42(9):1695-1701. doi: 10.1002/jssc.201900061. Epub 2019 Mar 18.
The simultaneous quantification of two potential genotoxic hydroxymethyl furan derivatives in coffee (furfuryl alcohol and 5-hydroxymethylfurfural) alongside their carboxylic acid derivatives (2-furoic acid and 5-hydroxymethyl furoic acid, respectively) was carried out. Their extraction from ground roasted coffee using sonication, simple shaking or heat-assisted shaking lead to similar results. A minimum of 97.3% of the four furan derivatives were extracted during the first extraction cycle using water, whereas methanol showed considerably lower extraction efficiency. A simple high-performance liquid chromatography method coupled with diode array detection was developed for the simultaneous determination and was applied to roasted coffee extracts or brews. No sample pre-treatment except for centrifugation was needed. The diode array detector was used to assess the purity of the peaks of interest in analyzed samples against authentic standards. The linearity according to Mandel, accuracy (recovery ≥ 89.9%) and precision (inter- and intraday relative standard deviation ≤ 4.5%) were checked. The values for the limit of detection and quantification ranged within 0.11-0.76 and 0.35-2.55 μg/mL, respectively. Filtered and espresso brews were analyzed for the four furan derivatives where furfuryl alcohol showed double the concentration of 5-hydroxymethylfurfural and about ten times the concentrations of 2-furoic acid or 5-hydroxymethyl furoic acid.
同时定量分析咖啡中的两种潜在遗传毒性羟甲基糠醛衍生物（糠醇和 5-羟甲基糠醛）及其羧酸衍生物（分别为 2-糠酸和 5-羟甲基糠酸）。使用超声、简单摇晃或热辅助摇晃从研磨的烤咖啡中提取这四种糠醛衍生物，结果相似。在第一个提取循环中，使用水可提取至少 97.3%的四种糠醛衍生物，而甲醇的提取效率则低得多。建立了一种简单的高效液相色谱法结合二极管阵列检测法，用于同时测定并应用于烤咖啡提取物或冲泡物。除了离心之外，不需要进行样品预处理。二极管阵列检测器用于评估分析样品中感兴趣的峰的纯度，以与真实标准相对照。根据 Mandel 进行了线性度检查、准确性（回收率≥89.9%）和精密度（日内和日间相对标准偏差≤4.5%）。检测限和定量限的值分别在 0.11-0.76 和 0.35-2.55μg/mL 范围内。分析了过滤咖啡和浓咖啡冲泡物中的四种糠醛衍生物，其中糠醇的浓度是 5-羟甲基糠醛的两倍，2-糠酸或 5-羟甲基糠酸的浓度则分别是其十倍。
