[A simplified quick semi-quantitative determination of urinary estrogen in non-pregnant female: the application for monitoring of follicular maturation at HMG-HCG therapy].

We have developed a simplified quick semiquantitative determination for urinary estrogen in non-pregnant female by utilizing Amberlite XAD-2 and E3 LAIR-KIT. The whole procedure required only 20 minutes. Interassay variance of this method was 10% and intraassay variance was 7%. The recovery was 85%. There was a highly significant correlation between the data obtained by this method and those by RIA (r = 0.944). We applied this method to the monitoring of follicular maturation in the HMG-HCG therapy. Urinary estrogens obtained from 28 anovulatory women (60 cycles) were determined by this method in order to decide the appropriate timing for the HCG injection following the HMG. Our data indicate that it is possible to induce ovulation without the risk of ovarian hyperstimulation syndrome if the HCG was injected on the day when the urinary estrogen level rose to between 70 and 100 ng/ml for 2 consecutive days. This method could well be applied to prospective monitoring in ovulation induction with HMG-HCG therapy.