A Relationship Between O-Serotype, Antibiotic Susceptibility and Biofilm Formation in Uropathogenic .

Uropathogenic (UPEC) is a well-known pathogen that has perturbed the medical scenario because of its resistance to diverse therapeutic drugs and its ability to form a biofilm. Different O-serogroups are the prevalent cause of urinary tract infections (UTIs) along with their ability to form a biofilm. The present research aimed to assess antibiotic susceptibility, biofilm formation, and serotyping of UPEC isolates in conjunction with the demographic data. Antibiotic susceptibility was determined using the Kirby-Bauer method and biofilm formation was assessed phenotypically and at the molecular level. Serotyping was performed by multiplex PCR. A significant proportion of the total of 120 UPECs was isolated from women ( < 0.05). Most isolates were resistant to cefotaxime, ceftazidime, and tetracycline, but maintained their sensitivity to imipenem. O, O, O, and O were the most commonly detected serogroups. Moreover, O, O and O were the highest biofilm-producing serogroups among the UPEC isolates. Serogroups O and O were significantly associated with diabetic patients and subjects with renal disease, respectively ( < 0.05). Overall, our results show that UTI incidence in women should be a subject of concern. The high prevalence of the O serogroup associated with a specific antibiotic profile and a high percentage of biofilm formation suggests a close relation between serogroups and characteristic features of UPEC isolates.