Xu Yahuan, Shao Bibo
Department of Cardiothoracic Surgery.
Department of Intensive Care Unit, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, China.
Medicine (Baltimore). 2019 Mar;98(9):e14558. doi: 10.1097/MD.0000000000014558.
This study aimed to investigate the correlation of long noncoding RNA (lncRNA) ZNFX1 antisense RNA (ZFAS1) with disease risk, severity, inflammation markers, and prognosis in sepsis patients.A total of 202 sepsis patients were consecutively enrolled, and 200 healthy volunteers were also recruited as healthy controls (HCs). Plasma samples of all patients and HCs were collected. LncRNA ZFAS1 expression was determined by quantitative polymerase chain reaction assay and inflammatory cytokines levels were detected by enzyme-linked immunosorbent assay.The median value of lncRNA ZFAS1 expression in sepsis patients was (0.639 [0.325-1.071]), which was lower compared to HCs (1.957 [0.876-3.245], P < .001], and receiver operating characteristics (ROC) curve revealed that lncRNA ZFAS1 expression had a good diagnostic value for sepsis with area under curve (AUC) of 0.814 (95% confidence interval [CI]: 0.771-0.857). Spearman test disclosed that lncRNA ZFAS1 expression was negatively correlated with Acute Physiology and Chronic Health Evaluation (APACHE) II score (r = -0.505, P < .001), and it was negatively associated with levels of C-creative protein (r = -0.241, P = .001), tumor necrosis factor-α (r = -0.253, P < .001), and interleukin (IL)-6 (r = -0.177, P = .012) while positively associated with IL-10 level (r = 0.173, P = .014). Also, lncRNA ZFAS1 expression was lower in survivor group compared to nonsurvivor group (P < .001), and it presented with a good predictive value on distinguishing nonsurvivors from survivors in sepsis patients with AUC of 0.628 (95% CI: 0.538-0.717).Circulating lncRNA ZFAS1 expression negatively correlates with disease risk, severity, and inflammatory markers levels, and might predict worse survival in sepsis patients.
本研究旨在探讨长链非编码RNA（lncRNA）锌指蛋白X1反义RNA（ZFAS1）与脓毒症患者疾病风险、严重程度、炎症标志物及预后的相关性。共连续纳入202例脓毒症患者，并招募200名健康志愿者作为健康对照（HCs）。收集所有患者及HCs的血浆样本。通过定量聚合酶链反应测定法测定lncRNA ZFAS1表达，采用酶联免疫吸附测定法检测炎性细胞因子水平。脓毒症患者lncRNA ZFAS1表达的中位数为（0.639[0.325 - 1.071]），低于HCs（1.957[0.876 - 3.245]，P＜0.001），受试者工作特征（ROC）曲线显示lncRNA ZFAS1表达对脓毒症具有良好的诊断价值，曲线下面积（AUC）为0.814（95%置信区间[CI]：0.771 - 0.857）。Spearman检验显示lncRNA ZFAS1表达与急性生理与慢性健康状况评分系统（APACHE）Ⅱ评分呈负相关（r = - 0.505，P＜0.001），与C反应蛋白水平（r = - 0.241，P = 0.001）、肿瘤坏死因子-α（r = - 0.253，P＜0.001）及白细胞介素（IL）-6（r = - 0.177，P = 0.012）呈负相关，而与IL - 10水平呈正相关（r = 0.173，P = 0.014）。此外，存活组lncRNA ZFAS1表达低于非存活组（P＜0.001），其在区分脓毒症患者非存活者与存活者方面具有良好的预测价值，AUC为0.628（95%CI：0.538 - 0.717）。循环lncRNA ZFAS1表达与疾病风险、严重程度及炎症标志物水平呈负相关，可能预测脓毒症患者更差的生存情况。
