Ansorg R A, Zarifoglu F I
Med Microbiol Immunol. 1986;174(6):305-12. doi: 10.1007/BF02123683.
Different culture conditions, devitalizing treatments, and preservation procedures were tested for the production of protein A-bearing cells of Staphylococcus aureus ATCC 12598. Native cells with the highest IgG-binding activity were obtained after cultivation at 37 degrees C for 24-48 h in enriched media. Devitalization by ethanol, 1-propanol, formaldehyde, glutardialdehyde, chloramine T, and by heat, reduced the protein A activity depending on the duration of treatment. The protein A content of devitalized cells were best preserved by storage at -20 degrees C or by lyophilization. Staphylococcal preparations with a stable IgG-binding activity of 20 mg/g bacteria, which are very suitable for coagglutination tests, were produced by culture in TSB medium, followed by devitalization with 1-propanol and lyophilization.
对不同的培养条件、灭活处理和保存程序进行了测试，以生产金黄色葡萄球菌ATCC 12598的含蛋白A细胞。在富集培养基中于37℃培养24 - 48小时后，可获得具有最高IgG结合活性的天然细胞。乙醇、1 - 丙醇、甲醛、戊二醛、氯胺T以及加热灭活，会根据处理时间降低蛋白A活性。灭活细胞的蛋白A含量通过在-20℃储存或冻干能得到最佳保存。通过在TSB培养基中培养，随后用1 - 丙醇灭活并冻干，可制备出具有20 mg/g细菌稳定IgG结合活性的葡萄球菌制剂，这种制剂非常适合用于协同凝集试验。
