Virology Department, National Reference Laboratory of Measles, Mumps, Rubella, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba.
Department of Infection and Immunity, Infectious Diseases Research Unit, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg.
J Med Virol. 2019 Jul;91(7):1351-1354. doi: 10.1002/jmv.25444. Epub 2019 Mar 14.
Between September 2014 and December 2015, 298 sera from rash and fever patients from all over Cuba were investigated for specific IgM antibodies against measles, rubella, dengue, human parvovirus B19 (B19V) and human herpesvirus 6 (HHV6) using a commercial enzyme-linked immunosorbent assay kits. B19V IgM positive and equivocal samples were investigated by a polymerase chain reaction and genotyping. No measles, rubella or dengue cases were detected. HHV6-IgM antibodies were confirmed in 5.7% and B19V-IgM antibodies in 10.7% of the patients. A total of 31.3% of the B19V cases were between 5 and 9 years old and 34.4% were 20 years and older. The only B19V sequence obtained belonged to genotype 1a. Diagnosis was established for only 16% of the rash and fever patients, suggesting that other diseases such as Zika or Chikungunya may play a role.
2014 年 9 月至 2015 年 12 月，使用商业酶联免疫吸附试验试剂盒检测了来自古巴各地的 298 例皮疹和发热患者的血清中针对麻疹、风疹、登革热、人细小病毒 B19（B19V）和人疱疹病毒 6（HHV6）的特异性 IgM 抗体。对 B19V IgM 阳性和可疑样本进行聚合酶链反应和基因分型检测。未检测到麻疹、风疹或登革热病例。HHV6-IgM 抗体在 5.7%的患者中得到确认，B19V-IgM 抗体在 10.7%的患者中得到确认。B19V 病例中，共有 31.3%的患者年龄在 5 至 9 岁之间，34.4%的患者年龄在 20 岁及以上。仅获得的一个 B19V 序列属于基因型 1a。仅对 16%的皮疹和发热患者做出了诊断，这表明其他疾病，如寨卡病毒或基孔肯雅热，可能也发挥了作用。
