Wawina Tony Bokalanga, Tshiani Olivier Mbaya, Ahuka Steve Mundeke, Pukuta Elisabeth Simbu, Aloni Michel Ntetani, Kasanga Christopher Jacob, Muyembe Jean-Jacques Tamfum
KU Leuven, Department of Microbiology and Immunology, Laboratory of Clinical and Epidemiological Virology (Rega Institute), Herestraat 49, B-3000 Leuven, Belgium; University Hospital of Kinshasa, Faculty of Medicine, Department of Microbiology, Kinshasa, Congo.
University Hospital of Kinshasa, Faculty of Medicine, Department of Microbiology, Kinshasa, Congo.
Int J Infect Dis. 2017 Dec;65:4-7. doi: 10.1016/j.ijid.2017.09.018. Epub 2017 Sep 22.
It has been demonstrated that infection with human parvovirus B19 (B19V) is associated with rash-fever illnesses. The present study aimed to investigate B19V as an aetiological agent of rash-fever syndromes in Congolese children confirmed as measles and rubella IgM-negative. An ELISA IgM test and PCR were performed to screen for B19V.
A total of 177 archived serum samples were randomly selected from the measles biobank of the National Institute for Biomedical Research (INRB). Samples were investigated for anti-B19V IgM and B19V DNA. These samples originated from children <5years of age with measles-like rashes, previously confirmed as negative for both measles and rubella IgM.
Out of 177 serum samples tested by ELISA and 168 tested by PCR, 109 were positive for B19V IgM antibodies (61.6%) and 87 (51.8%) were positive for B19V DNA. Positive samples in both assays were from all provinces of DRC.
B19V plays a role in rash-fever illnesses in children under 5 years of age suspected of having measles or rubella infections in DRC. As an aetiological cause of rash and fever syndromes, the present study demonstrates that B19V should also be considered during the laboratory investigation of rash-fever illnesses in DRC, particularly in the paediatric population. There is a need to conduct further studies in order to gain a better understanding of the spatiotemporal pattern of B19V and to define the genotype(s) of B19V circulating in DRC.
已证实感染人细小病毒B19（B19V）与皮疹热疾病有关。本研究旨在调查B19V作为刚果儿童皮疹热综合征的病原体，这些儿童麻疹和风疹IgM检测呈阴性。采用酶联免疫吸附试验（ELISA）IgM检测和聚合酶链反应（PCR）对B19V进行筛查。
从国家生物医学研究所（INRB）的麻疹生物样本库中随机选取177份存档血清样本。对样本进行抗B19V IgM和B19V DNA检测。这些样本来自5岁以下出现麻疹样皮疹的儿童，之前麻疹和风疹IgM检测均为阴性。
在177份经ELISA检测的血清样本和168份经PCR检测的样本中，109份B19V IgM抗体呈阳性（61.6%），87份（51.8%）B19V DNA呈阳性。两种检测均为阳性的样本来自刚果民主共和国的所有省份。
在刚果民主共和国，B19V在疑似感染麻疹或风疹的5岁以下儿童的皮疹热疾病中起作用。作为皮疹和发热综合征的病因，本研究表明，在刚果民主共和国对皮疹热疾病进行实验室调查时，尤其是对儿科人群，也应考虑B19V。有必要开展进一步研究以便更好地了解B19V的时空模式，并确定在刚果民主共和国传播的B19V基因型。
