Laboratorio de Fitofarmacología, Dirección de Investigaciones en Neurociencias. Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada México-Xochimilco 101, Col. San Lorenzo Huipulco, Tlalpan, 14370 CDMX, Mexico.
Laboratorio de Biología de la Reproducción, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Col. Insurgentes Cuicuilco, Coyoacán, 04530 CDMX, Mexico.
J Ethnopharmacol. 2019 May 23;236:50-62. doi: 10.1016/j.jep.2019.02.036. Epub 2019 Feb 25.
To explore the antidepressant- and anxiolytic-like effects of an aqueous extract of Turnera diffusa Willd (Turneraceae) and to explore its possible toxic side effects on behavior, target organ function, and spermatic quality.
Acute effects of a T. diffusa aqueous extract were evaluated in adult male mice with the plus-maze, forced swimming and open field tests to identify the possible anxiolytic, antidepressant and stimulant effects of this extract. Effects of T. diffusa aqueous extract were further investigated through two approaches. a) Male and female adult mice receiving a 28-day treatment were evaluated in a neurobehavioral test battery; later, changes in their biochemical parameters and in target organ morphology were analyzed. b) In young adult (16-weeks old) and mature (46-weeks old) males, spermatic quality and testes morphology during a complete spermatogenesis cycle were analyzed after a 35-day treatment.
T. diffusa aqueous extract induced remarkable anxiolytic- and antidepressant-like effects without affecting locomotor activity. This extract did not elicit behavioral signs of neural side effects, a sex-dependent reduction in body weight gain was produced without affecting functional parameters or the morphology of target organs. The highest dose improved cellular turnover in the testes of mature mice.
T. diffusa aqueous extract induced a clear anxiolytic-like effect, and for the first time, we reported an antidepressant effect. Clinical potential or even intake of T. diffusa in the context of traditional medicine can be supported by its efficacy to positively modulate behavior and its safety for a wide range of doses.
探索 Turnera diffusa Willd（Turneraceae）水提物的抗抑郁和抗焦虑样作用，并探索其对行为、靶器官功能和精子质量可能产生的毒副作用。
通过高架十字迷宫、强迫游泳和旷场试验评估 T. diffusa 水提物的急性作用，以确定该提取物可能具有的抗焦虑、抗抑郁和兴奋剂作用。通过两种方法进一步研究 T. diffusa 水提物的作用：a）接受 28 天治疗的雄性和雌性成年小鼠进行神经行为测试；然后分析其生化参数和靶器官形态的变化。b）在年轻成年（16 周龄）和成熟（46 周龄）雄性小鼠中，在 35 天治疗后分析完整精子发生周期中的精子质量和睾丸形态。
T. diffusa 水提物诱导出明显的抗焦虑和抗抑郁样作用，而不影响运动活性。该提取物不会引起神经副作用的行为迹象，会产生性别依赖性的体重增加减少，但不影响功能参数或靶器官的形态。最高剂量可改善成熟小鼠睾丸中的细胞更新。
T. diffusa 水提物诱导出明显的抗焦虑样作用，并且我们首次报道了其具有抗抑郁作用。在传统医学的背景下，T. diffusa 的临床潜力甚至摄入可以得到其对行为的积极调节作用及其在广泛剂量范围内的安全性的支持。
