Novas M Victoria, Cabral Daniel
Depto. de Cs. Biologicas, FCEN, Universidad de Buenos Aires, Ciudad Universitaria, 1428 EHA, Buenos Aires, Argentina.
Plant Dis. 2002 Mar;86(3):215-219. doi: 10.1094/PDIS.2002.86.3.215.
Vegetative compatibility (VC) of Aspergillus flavus isolates from peanut seed was studied to evaluate preliminary diversity and its association with mycotoxin production and sclerotia production and number. A. parasiticus isolates also were included as a comparative group. Isolates were divided into five categories based on mycotoxin production combination. Five of the A. flavus isolates were considered atypical because they simultaneously produced aflatoxins B, G, and cyclopiazonic acid (CPA). Vegetative compatibility groups (VCGs) were determined through complementation tests between nitrate-nonutilizing mutants. Sclerotia diameters and the number of sclerotia produced per square centimeter were determined for each isolate. Out of 32 isolates of A. flavus, 25 combined in 13 VCGs, whereas the remaining could not be assigned to any particular group. Each VCG included isolates of the same mycotoxin category, with only one exception. Also, all isolates within the same VCG were characterized by their ability to produce or not produce sclerotia. Isolates between VCGs showed significant differences in number of sclerotia per square centimeter, but differences in sclerotia size were not evident. Atypical isolates simultaneously producing aflatoxins B, G, and CPA formed a single and exclusive VCG.
对来自花生种子的黄曲霉分离株的营养体亲和性（VC）进行了研究，以初步评估其多样性及其与霉菌毒素产生、菌核产生和数量的关系。寄生曲霉分离株也作为一个比较组纳入研究。根据霉菌毒素产生组合，将分离株分为五类。五株黄曲霉分离株被认为是非典型的，因为它们同时产生黄曲霉毒素B、G和环匹阿尼酸（CPA）。通过硝酸盐非利用突变体之间的互补试验确定营养体亲和群（VCGs）。测定了每个分离株的菌核直径和每平方厘米产生的菌核数量。在32株黄曲霉分离株中，25株组合成13个VCGs，其余的无法归入任何特定组。每个VCG都包含同一霉菌毒素类别的分离株，只有一个例外。此外，同一VCG内的所有分离株都以其产生或不产生菌核的能力为特征。不同VCG之间的分离株在每平方厘米菌核数量上存在显著差异，但菌核大小差异不明显。同时产生黄曲霉毒素B、G和CPA的非典型分离株形成了一个单一且独特的VCG。
