Evaluation of Corn Inbreds and Advanced Breeding Lines for Resistance to Aflatoxin Contamination in the Field.

Eighteen corn inbred lines and advanced breeding lines were evaluated for resistance to aflatoxin contamination when artificially inoculated with Aspergillus flavus in 1998, 1999 (two tests), and 2000 at Mississippi State, MS, in field studies. The top ear of each plant was inoculated with the A. flavus isolate NRRL 3357 seven days after midsilk (50% of the plants in a plot had silks emerged) using the side-needle technique. Ears were harvested at kernel maturity approximately 63 days after midsilk and aflatoxin levels were measured using the Vicam AflaTest. Aflatoxin contamination in the inbreds was extremely high in 1998. Levels ranged from 139 to 21,090 ng/g. In 1999, aflatoxin contamination ranged from 17 to 1,070 ng/g in one test and 14 to 1,278 ng/g in another test. In 2000, aflatoxin levels ranged from 237 to 7,503 ng/g. Lines that supported lowest levels of aflatoxin contamination included Mp81:112, Mp92:673, Mp92:679, and Mp494. These lines provide potential new sources of resistance that can be used to move aflatoxin resistance into commercial corn hybrids.