伊朗花生芽坏死病毒的首次报道。

First Report of Groundnut bud necrosis virus in Iran.

作者信息

Golnaraghi A R, Pourrahim R, Shahraeen N, Farzadfar Sh

机构信息

Plant Virology Department, Plant Pests and Diseases Research Institute, P. O. Box 19395-1454, Tehran, Iran.

出版信息

Plant Dis. 2002 May;86(5):561. doi: 10.1094/PDIS.2002.86.5.561B.

DOI:10.1094/PDIS.2002.86.5.561B

PMID:30818693

Abstract

During the summer of 2001, mosaic, mottle, ring mosaic, stunting, and bud necrosis were observed in peanut fields (Arachis hypogaea cv. Gilan) in the Golestan Province of Iran. Mechanical inoculation of these samples caused necrotic local lesions on Vigna unguiculata cv. Mashad, necrosis on Nicotiana benthamiana and N. rustica, and mosaic followed by bud necrosis on Arachis hypogaea cv. NC2. Using triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and polyclonal (As) combined with monoclonal antibodies (MAbs) produced by DSMZ (Braunschweig, Germany), the samples were tested for presence of impatiens necrotic spot virus (INSV) (As-0115, MAb-0117-5E4), tomato spotted wilt virus (TSWV) (As-0105, MAb-0116-2B6, MAb-0106-4F2), and groundnut bud necrosis virus (GBNV) (As-0118, MAb-0226-1B4). The samples also were checked by TSWV polyclonal antibody (As-0526, As-0580, DSMZ). ELISA results showed leaf samples and inoculated indicator plants reacted positively to GBNV antibodies. Also a weak reaction was observed with TSWV-polyclonal antibody. However no reaction was detected using the INSV and TSWV-MAbs. GBNV is a member of the Tospovirus genus and has serological relationship with TSWV (1). To our knowledge, this is the first report of GBNV occurrence in Iran. Reference: (1) C. Heinze et al. Phytopathology 85:683-690, 1995.

摘要

2001年夏季，在伊朗戈勒斯坦省的花生田（落花生品种吉兰）中观察到花叶、斑驳、环斑花叶、矮化和芽坏死现象。对这些样本进行机械接种后，在豇豆品种马什哈德上引起坏死性局部病斑，在本氏烟草和黄花烟草上引起坏死，在花生品种NC2上引起花叶随后芽坏死。使用三抗体夹心酶联免疫吸附测定（TAS - ELISA）以及德国不伦瑞克的德国微生物和细胞培养物保藏中心（DSMZ）生产的多克隆抗体（As）与单克隆抗体（MAb）相结合，对样本检测凤仙花坏死斑病毒（INSV）（As - 0115，MAb - 0117 - 5E4）、番茄斑萎病毒（TSWV）（As - 0105，MAb - 0116 - 2B6，MAb - 0106 - 4F2）和花生芽坏死病毒（GBNV）（As - 0118，MAb - 0226 - 1B4）的存在情况。样本还通过TSWV多克隆抗体（As - 0526，As - 0580，DSMZ）进行检测。ELISA结果显示叶片样本和接种的指示植物对GBNV抗体呈阳性反应。使用TSWV多克隆抗体也观察到微弱反应。然而，使用INSV和TSWV单克隆抗体未检测到反应。GBNV是番茄斑萎病毒属的成员，与TSWV有血清学关系（1）。据我们所知，这是GBNV在伊朗发生的首次报道。参考文献：（1）C. Heinze等人，《植物病理学》85:683 - 690，1995年。