腺苷酸环化酶的GTP结合刺激性蛋白α亚基的推导一级结构。
Deduced primary structure of the alpha subunit of the GTP-binding stimulatory protein of adenylate cyclase.
作者信息
Robishaw J D, Russell D W, Harris B A, Smigel M D, Gilman A G
出版信息
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1251-5. doi: 10.1073/pnas.83.5.1251.
Abstract
A bovine adrenal cDNA clone encoding the entire alpha subunit of the GTP-binding regulatory protein that stimulates adenylate cyclase (Gs) was isolated and sequenced. This cDNA directed the synthesis of the larger, 52-kDa form of the polypeptide in COS cells, even though the clone appeared to encode a 46-kDa protein. Comparison of the deduced amino acid sequence of Gs alpha with the alpha subunit of another G protein, transducin, revealed striking homologies.
摘要
分离并测序了一个牛肾上腺cDNA克隆，该克隆编码刺激腺苷酸环化酶（Gs）的GTP结合调节蛋白的整个α亚基。尽管该克隆似乎编码一种46 kDa的蛋白质，但此cDNA在COS细胞中指导合成了较大的、52 kDa形式的多肽。将Gsα推导的氨基酸序列与另一种G蛋白转导素的α亚基进行比较，发现了显著的同源性。
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本文引用的文献
1
The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence.大肠杆菌延伸因子Tu的氨基酸序列。完整序列。
J Biol Chem. 1981 Aug 10;256(15):8102-9.
2
The nucleotide sequence of the cloned tufA gene of Escherichia coli.大肠杆菌克隆的tufA基因的核苷酸序列。
Gene. 1980 Dec;12(1-2):25-31. doi: 10.1016/0378-1119(80)90012-8.
3
Establishing homologies in protein sequences.确定蛋白质序列中的同源性。
Methods Enzymol. 1983;91:524-45. doi: 10.1016/s0076-6879(83)91049-2.
4
Amino acid sequence of retinal transducin at the site ADP-ribosylated by cholera toxin.视网膜转导蛋白在被霍乱毒素进行ADP-核糖基化修饰位点的氨基酸序列。
J Biol Chem. 1984 Jan 25;259(2):696-8.
5
Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain.从牛脑细胞膜中分离出两种对鸟嘌呤核苷酸具有高亲和力的蛋白质。
J Biol Chem. 1984 Nov 25;259(22):13806-13.
6
Homologies between signal transducing G proteins and ras gene products.信号转导G蛋白与ras基因产物之间的同源性。
Science. 1984 Nov 16;226(4676):860-2. doi: 10.1126/science.6436980.
7
Regional homology in GTP-binding proto-oncogene products and elongation factors.GTP结合原癌基因产物与延伸因子中的区域同源性。
J Cyclic Nucleotide Protein Phosphor Res. 1983;9(6):435-48.
8
Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.酿酒酵母中编码与哺乳动物ras蛋白具有同源结构域的蛋白质的基因。
Cell. 1984 Mar;36(3):607-12. doi: 10.1016/0092-8674(84)90340-4.
9
Mechanisms of guanine nucleotide-mediated regulation of adenylate cyclase activity.鸟嘌呤核苷酸介导的腺苷酸环化酶活性调节机制。
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10
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J Biol Chem. 1984 Mar 25;259(6):3560-7.
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