Hajjar J J, Van Linda B, Fucci J, Tomicic T
Prostaglandins. 1986 Jan;31(1):83-93. doi: 10.1016/0090-6980(86)90227-3.
Intestinal obstruction inhibits amino acid absorption. The inhibition, being dependent on the pathological changes of the absorptive epithelium, was considered as an index of injury and measured after varying periods of obstruction and after pretreatment with clindamycin, indomethacin, 16,16-dimethyl-PGE2 or arachidonic acid. A reduction in amino acid uptake was apparent after 2h of obstruction and was increasingly evident after 4, 6 and 18 h. During the late phase (after 6 h), inhibition was partly prevented by pretreatment with clindamycin, but the antibiotic was ineffective during the early phase (within the first 2 h). Bacterial colony counts of luminal contents of rats obstructed for 2 h, were not different from counts obtained in controls, but significantly lower than counts in rats that have been obstructed for 6 h. Pretreatment of rats with 16,16-dimethyl-PGE2 or with arachidonic acid prevented the early inhibitory effects of the obstruction. The findings suggest that the early inhibition in amino acid uptake may be related to metabolic changes that are correctable by the administration of 16,16-dimethyl-PGE2 or of arachidonic acid. The inhibition, during the late phase, is mainly related to an overgrowth of the enteric bacteria.
肠梗阻会抑制氨基酸吸收。这种抑制作用取决于吸收上皮的病理变化，被视为损伤指标，并在不同时间段的肠梗阻后以及用克林霉素、吲哚美辛、16,16 - 二甲基前列腺素E2或花生四烯酸预处理后进行测量。梗阻2小时后氨基酸摄取明显减少，4、6和18小时后愈发明显。在后期（6小时后），克林霉素预处理可部分防止抑制作用，但抗生素在早期（最初2小时内）无效。梗阻2小时的大鼠肠腔内容物的细菌菌落计数与对照组无差异，但显著低于梗阻6小时的大鼠。用16,16 - 二甲基前列腺素E2或花生四烯酸预处理大鼠可防止梗阻的早期抑制作用。研究结果表明，氨基酸摄取的早期抑制可能与可通过给予16,16 - 二甲基前列腺素E2或花生四烯酸纠正的代谢变化有关。后期的抑制主要与肠道细菌过度生长有关。
