Ebe Shohei, Ohike Tatsuya, Matsukawa Tetsuya, Okanami Masahiro, Kajiyama Shin'ichiro, Ano Takashi
Division of Biotechnological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan.
J Pestic Sci. 2019 Feb 20;44(1):33-40. doi: 10.1584/jpestics.D18-042.
The purpose of this study is to isolate the beneficial microorganisms whose growth is promoted in the presence of charcoal materials. We successfully isolated strain IA, whose growth is promoted on an agar plate with charcoal materials, and identified it as a novel strain of the sp. The growth of strain IA in the liquid medium was promoted by the addition of both activated charcoal (AC) and rice husk biochar (RHB). Moreover, the sporulation of strain IA in the RHB medium and the antifungal activity of the culture supernatant of the RHB medium were much higher than those with AC. HPLC and MS analyses revealed that strain IA produced an antifungal lipopeptide iturin A, and the yield of iturin A in the RHB medium was 8 times higher than that in the medium without RHB. This is the first paper to describe the positive effect of RHB on microbial metabolisms.
本研究的目的是分离出在木炭材料存在下生长得到促进的有益微生物。我们成功分离出了菌株IA，其在含有木炭材料的琼脂平板上生长得到促进，并将其鉴定为 sp. 的一个新菌株。在液体培养基中，添加活性炭（AC）和稻壳生物炭（RHB）均能促进菌株IA的生长。此外，菌株IA在RHB培养基中的孢子形成以及RHB培养基培养上清液的抗真菌活性均远高于添加AC的情况。高效液相色谱（HPLC）和质谱（MS）分析表明，菌株IA产生了一种抗真菌脂肽伊枯草菌素A，且RHB培养基中伊枯草菌素A的产量比无RHB培养基中的产量高8倍。本文首次描述了RHB对微生物代谢的积极影响。
